Abstract
The uvrD gene product apparently plays a role in the repair of UV damage, in mismatch repair, and in genetic recombination. A lower level of expression of the Salmonella typhimurium LT2 uvrD gene was observed in maxicells prepared from an Escherichia coli strain that contained a lexA+ plasmid than in maxicells prepared from an E. coli strain that lacked functional LexA protein. These results suggest that the uvrD+ gene is repressed by the LexA protein and is thus a member of the set of genes whose expression is increased by "SOS"-inducing treatments.
Full text
PDF![1502](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd6/217635/dd799d6f2134/jbacter00247-0480.png)
![1503](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd6/217635/0e6a7ca09490/jbacter00247-0481.png)
![1504](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd6/217635/2e279fdbe73b/jbacter00247-0482.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bagg A., Kenyon C. J., Walker G. C. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749–5753. doi: 10.1073/pnas.78.9.5749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brent R., Ptashne M. The lexA gene product represses its own promoter. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932–1936. doi: 10.1073/pnas.77.4.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dobson P. P., Walker G. C. Plasmid (pKM101)-mediated Weigle reactivation in Escherichia coli K12 and Salmonella typhimurium LT2: genetic dependence, kinetics of induction, and effect of chloramphenicol. Mutat Res. 1980 Jun;71(1):25–41. doi: 10.1016/0027-5107(80)90004-4. [DOI] [PubMed] [Google Scholar]
- Elledge S. J., Walker G. C. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. J Mol Biol. 1983 Feb 25;164(2):175–192. doi: 10.1016/0022-2836(83)90074-8. [DOI] [PubMed] [Google Scholar]
- Fogliano M., Schendel P. F. Evidence for the inducibility of the uvrB operon. Nature. 1981 Jan 15;289(5794):196–198. doi: 10.1038/289196a0. [DOI] [PubMed] [Google Scholar]
- Glickman B. W., Radman M. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063–1067. doi: 10.1073/pnas.77.2.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glickman B., van den Elsen P., Radman M. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance. Mol Gen Genet. 1978 Jul 25;163(3):307–312. doi: 10.1007/BF00271960. [DOI] [PubMed] [Google Scholar]
- Huisman O., D'Ari R. An inducible DNA replication-cell division coupling mechanism in E. coli. Nature. 1981 Apr 30;290(5809):797–799. doi: 10.1038/290797a0. [DOI] [PubMed] [Google Scholar]
- Kenyon C. J., Walker G. C. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci U S A. 1980 May;77(5):2819–2823. doi: 10.1073/pnas.77.5.2819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. doi: 10.1016/0092-8674(82)90085-x. [DOI] [PubMed] [Google Scholar]
- Maples V. F., Kushner S. R. DNA repair in Escherichia coli: identification of the uvrD gene product. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5616–5620. doi: 10.1073/pnas.79.18.5616. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller H. I., Kirk M., Echols H. SOS induction and autoregulation of the himA gene for site-specific recombination in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6754–6758. doi: 10.1073/pnas.78.11.6754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nevers P., Spatz H. C. Escherichia coli mutants uvr D and uvr E deficient in gene conversion of lambda-heteroduplexes. Mol Gen Genet. 1975 Aug 27;139(3):233–243. doi: 10.1007/BF00268974. [DOI] [PubMed] [Google Scholar]
- Oeda K., Horiuchi T., Sekiguchi M. Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency. Mol Gen Genet. 1981;184(2):191–199. doi: 10.1007/BF00272904. [DOI] [PubMed] [Google Scholar]
- Pang P. P., Walker G. C. Identification of the uvrD gene product of Salmonella typhimurium LT2. J Bacteriol. 1983 Mar;153(3):1172–1179. doi: 10.1128/jb.153.3.1172-1179.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perry K. L., Walker G. C. Identification of plasmid (pKM101)-coded proteins involved in mutagenesis and UV resistance. Nature. 1982 Nov 18;300(5889):278–281. doi: 10.1038/300278a0. [DOI] [PubMed] [Google Scholar]
- Sancar A., Sancar G. B., Rupp W. D., Little J. W., Mount D. W. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature. 1982 Jul 1;298(5869):96–98. doi: 10.1038/298096a0. [DOI] [PubMed] [Google Scholar]
- Sancar A., Wharton R. P., Seltzer S., Kacinski B. M., Clarke N. D., Rupp W. D. Identification of the uvrA gene product. J Mol Biol. 1981 May 5;148(1):45–62. doi: 10.1016/0022-2836(81)90234-5. [DOI] [PubMed] [Google Scholar]
- Shanabruch W. G., Behlau I., Walker G. C. Spontaneous mutators of salmonella typhimurium LT2 generated by insertion of transposable elements. J Bacteriol. 1981 Sep;147(3):827–835. doi: 10.1128/jb.147.3.827-835.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shanabruch W. G., Rein R. P., Behlau I., Walker G. C. Mutagenesis, by methylating and ethylating agents, in mutH, mutL, mutS, and uvrD mutants of Salmonella typhimurium LT2. J Bacteriol. 1983 Jan;153(1):33–44. doi: 10.1128/jb.153.1.33-44.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tokuno S. I., Gough M. UV sensitivity of a nonrepressor regulatory protein of bacteriophage P22. J Virol. 1976 Apr;18(1):65–70. doi: 10.1128/jvi.18.1.65-70.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner R., Jr, Meselson M. Repair tracts in mismatched DNA heteroduplexes. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135–4139. doi: 10.1073/pnas.73.11.4135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White R. L., Fox M. S. On the molecular basis of high negative interference. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1544–1548. doi: 10.1073/pnas.71.4.1544. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wildenberg J., Meselson M. Mismatch repair in heteroduplex DNA. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2202–2206. doi: 10.1073/pnas.72.6.2202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]