Abstract
We determined the DNA sequence of the D-serine deaminase promoter region and of the N-terminal region of the structural gene. There are possibilities in the promoter for secondary structure and for initiation recognition sequences, and there is an open reading frame. The N-terminal sequence for the structural gene confirms that part of the amino acid sequence previously determined by E. Schlitz and W. Schmitt (FEBS Lett. 134:57-62, 1981), including the active site of the enzyme, and spans the two regions unresolved by their work.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bedouelle H., Hofnung M. A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12. Mol Gen Genet. 1982;185(1):82–87. doi: 10.1007/BF00333794. [DOI] [PubMed] [Google Scholar]
- Brown W. M., Watson R. M., Vinograd J., Tait K. M., Boyer H. W., Goodman H. M. The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12. Cell. 1976 Apr;7(4):517–530. doi: 10.1016/0092-8674(76)90202-6. [DOI] [PubMed] [Google Scholar]
- Carothers A. M., Heincz M. C., McFall E. Position effect on expression of dsd genes cloned onto multicopy plasmids. J Bacteriol. 1980 Apr;142(1):185–190. doi: 10.1128/jb.142.1.185-190.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carothers A. M., McFall E., Palchaudhuri S. Physical mapping of the Escherichia coli D-serine deaminase region: contiguity of the dsd structural and regulatory genes. J Bacteriol. 1980 Apr;142(1):174–184. doi: 10.1128/jb.142.1.174-184.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Debarbouille M., Cossart P., Raibaud O. A DNA sequence containing the control sites for gene malT and for the malPQ operon. Mol Gen Genet. 1982;185(1):88–92. doi: 10.1007/BF00333795. [DOI] [PubMed] [Google Scholar]
- Dowhan W., Jr, Snell E. E. D-serine dehydratase from Escherichia coli. II. Analytical studies and subunit structure. J Biol Chem. 1970 Sep 25;245(18):4618–4628. [PubMed] [Google Scholar]
- Heincz M. C., McFall E. N-terminal amino acid sequences of D-serine deaminases of wild-type and operator-constitutive strains of Escherichia coli K-12. J Bacteriol. 1975 Sep;123(3):1163–1168. doi: 10.1128/jb.123.3.1163-1168.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heincz M. C., McFall E. Role of the dsdC activator in regulation of D-serine deaminase synthesis. J Bacteriol. 1978 Oct;136(1):96–103. doi: 10.1128/jb.136.1.96-103.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee N. L., Gielow W. O., Wallace R. G. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Feb;78(2):752–756. doi: 10.1073/pnas.78.2.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Majors J. Initiation of in vitro mRNA synthesis from the wild-type lac promoter. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4394–4398. doi: 10.1073/pnas.72.11.4394. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McFall E., Heincz M. C. Identification and control of synthesis of the dsdC activator protein. J Bacteriol. 1983 Feb;153(2):872–877. doi: 10.1128/jb.153.2.872-877.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McFall E. Role of adenosine 3',5'-cyclic monophosphate and its specific binding protein in the regulation of D-serine deaminase synthesis. J Bacteriol. 1973 Feb;113(2):781–785. doi: 10.1128/jb.113.2.781-785.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palchaudhuri S., McFall E., Carothers A. M. Specialized transduction of D-serine deaminase genes: formation of lysogens that yield high lambda-d dsd/lambda ratios and formation of a dimeric lambda-d dsd. J Bacteriol. 1976 Aug;127(2):998–1014. doi: 10.1128/jb.127.2.998-1014.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Queen C., Rosenberg M. A promoter of pBR322 activated by cAMP receptor protein. Nucleic Acids Res. 1981 Jul 24;9(14):3365–3377. doi: 10.1093/nar/9.14.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
- Sanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975 May 25;94(3):441–448. doi: 10.1016/0022-2836(75)90213-2. [DOI] [PubMed] [Google Scholar]
- Schiltz E., Schmitt W. Sequence of Escherichia coli D-serine dehydratase. Location of the pyridoxal-phosphate binding site. FEBS Lett. 1981 Nov 2;134(1):57–62. doi: 10.1016/0014-5793(81)80550-9. [DOI] [PubMed] [Google Scholar]
