Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jul;155(1):169–179. doi: 10.1128/jb.155.1.169-179.1983

Purine salvage pathways of Bacillus subtilis and effect of guanine on growth of GMP reductase mutants.

T Endo, B Uratani, E Freese
PMCID: PMC217666  PMID: 6408059

Abstract

We have isolated numerous mutants containing mutations in the salvage pathways of purine synthesis. The mutations cause deficiencies in adenine phosphoribosyltransferase (adeF), in hypoxanthine-guanine phosphoribosyltransferase (guaF), in adenine deaminase (adeC), in inosine-guanosine phosphorylase, (guaP), and in GMP reductase (guaC). The physiological properties of mutants containing one or more of these mutations and corresponding enzyme measurements have been used to derive a metabolic chart of the purine salvage pathway of Bacillus subtilis.

Full text

PDF
169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berlin R. D., Stadtman E. R. A possible role of purine nucleotide pyrophosphorylases in the regulation of purine uptake by Bacillus subtilis. J Biol Chem. 1966 Jun 10;241(11):2679–2686. [PubMed] [Google Scholar]
  2. Coffee C. J. AMP deaminase from rat skeletal muscle. Methods Enzymol. 1978;51:490–497. doi: 10.1016/s0076-6879(78)51067-7. [DOI] [PubMed] [Google Scholar]
  3. Endo T., Ishihara H., Freese E. Properties of a Bacillus subtilis mutant able to sporulate continually during growth in synthetic medium. J Gen Microbiol. 1983 Jan;129(1):17–30. doi: 10.1099/00221287-129-1-17. [DOI] [PubMed] [Google Scholar]
  4. Freese E., Heinze J. E., Galliers E. M. Partial purine deprivation causes sporulation of Bacillus subtilis in the presence of excess ammonia, glucose and phosphate. J Gen Microbiol. 1979 Nov;115(1):193–205. doi: 10.1099/00221287-115-1-193. [DOI] [PubMed] [Google Scholar]
  5. Gots J. S., Benson C. E., Shumas S. R. Genetic separation of hypoxanthine and guanine-xanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium. J Bacteriol. 1972 Nov;112(2):910–916. doi: 10.1128/jb.112.2.910-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hochstadt J. Hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase from enteric bacteria. Methods Enzymol. 1978;51:549–558. doi: 10.1016/s0076-6879(78)51077-x. [DOI] [PubMed] [Google Scholar]
  7. Hoffee P. A., May R., Robertson B. D. Purine nucleoside phosphorylase from Salmonella typhimurium and rat liver. Methods Enzymol. 1978;51:517–524. doi: 10.1016/s0076-6879(78)51072-0. [DOI] [PubMed] [Google Scholar]
  8. Jensen K. F. Two purine nucleoside phosphorylases in Bacillus subtilis. Purification and some properties of the adenosine-specific phosphorylase. Biochim Biophys Acta. 1978 Aug 7;525(2):346–356. doi: 10.1016/0005-2744(78)90229-2. [DOI] [PubMed] [Google Scholar]
  9. Kalb V. F., Jr, Bernlohr R. W. A new spectrophotometric assay for protein in cell extracts. Anal Biochem. 1977 Oct;82(2):362–371. doi: 10.1016/0003-2697(77)90173-7. [DOI] [PubMed] [Google Scholar]
  10. Lopez J. M., Dromerick A., Freese E. Response of guanosine 5'-triphosphate concentration to nutritional changes and its significance for Bacillus subtilis sporulation. J Bacteriol. 1981 May;146(2):605–613. doi: 10.1128/jb.146.2.605-613.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lopez J. M., Marks C. L., Freese E. The decrease of guanine nucleotides initiates sporulation of Bacillus subtilis. Biochim Biophys Acta. 1979 Oct 4;587(2):238–252. doi: 10.1016/0304-4165(79)90357-x. [DOI] [PubMed] [Google Scholar]
  12. MAGER J., MAGASANIK B. Guanosine 5'-phosphate reductase and its role in the interconversion of purine nucleotides. J Biol Chem. 1960 May;235:1474–1478. [PubMed] [Google Scholar]
  13. Nishikawa H., Momose H., Shiio I. Pathway of purine nucleotide synthesis in Bacillus subtilis. J Biochem. 1968 Feb;63(2):149–155. doi: 10.1093/oxfordjournals.jbchem.a128755. [DOI] [PubMed] [Google Scholar]
  14. Nygaard P. Adenosine deaminase from Escherichia coli. Methods Enzymol. 1978;51:508–512. doi: 10.1016/s0076-6879(78)51070-7. [DOI] [PubMed] [Google Scholar]
  15. Ochi K., Kandala J. C., Freese E. Initiation of Bacillus subtilis sporulation by the stringent response to partial amino acid deprivation. J Biol Chem. 1981 Jul 10;256(13):6866–6875. [PubMed] [Google Scholar]
  16. Senesi S., Falcone G., Mura U., Sgarrella F., Ipata P. L. A specific adenosine phosphorylase, distinct from purine nucleoside phosphorylase. FEBS Lett. 1976 May 1;64(2):353–357. doi: 10.1016/0014-5793(76)80327-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES