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. 1983 Jul;155(1):357–366. doi: 10.1128/jb.155.1.357-366.1983

Sporulation of Streptomyces griseus in submerged culture.

K E Kendrick, J C Ensign
PMCID: PMC217688  PMID: 6134716

Abstract

A wild-type strain of Streptomyces griseus forms spores both on solid media (aerial spores) and in liquid culture (submerged spores). Both spore types are highly resistant to sonication, but only aerial spores are resistant to lysozyme digestion. Electron micrographs suggest that lysozyme sensitivity may result from the thinner walls of the submerged spores. Studies of the life cycle indicate that neither streptomycin excretion nor extracellular protease activity is required for sporulation: the analysis of mutants, however, suggests that antibiotic production may be correlated with the ability to sporulate. A method was devised to induce the rapid sporulation of S. griseus in a submerged culture. This method, which depends on nutrient deprivation, was used to determine that either ammonia or phosphate starvation can trigger sporulation and that the enzyme glutamine synthetase may be useful as a sporulation marker after phosphate deprivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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