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. 1983 Jul;155(1):412–416. doi: 10.1128/jb.155.1.412-416.1983

Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli.

D O Wood, W H Atkinson, R S Sikorski, H H Winkler
PMCID: PMC217695  PMID: 6345509

Abstract

Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. coli retains the regulatory control mechanisms characteristic of the rickettsial enzyme.

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Selected References

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