Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jul;155(1):443–446. doi: 10.1128/jb.155.1.443-446.1983

Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae.

M R Loeb, D H Smith
PMCID: PMC217703  PMID: 6345513

Abstract

Both inner and outer membrane proteins of Haemophilus influenzae type b were labeled by iodination procedures believed to be specific for exposed surface proteins only. It is suggested that this is due to specific properties of the outer membrane of H. influenzae and that use of these procedures with other gram-negative bacteria be evaluated carefully.

Full text

PDF
443

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer M. E. Structural and functional evidence of cooperativity between membranes and cell wall in bacteria. Int Rev Cytol Suppl. 1981;12:39–70. doi: 10.1016/b978-0-12-364373-5.50012-3. [DOI] [PubMed] [Google Scholar]
  2. Concino M. F., Goodgal S. H. Haemophilus influenzae polypeptides involved in deoxyribonucleic acid uptake detected by cellular surface protein iodination. J Bacteriol. 1981 Oct;148(1):220–231. doi: 10.1128/jb.148.1.220-231.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hansen E. J., Frisch C. F., McDade R. L., Jr, Johnston K. H. Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system. Infect Immun. 1981 Jun;32(3):1084–1092. doi: 10.1128/iai.32.3.1084-1092.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heckels J. E. The surface properties of Neisseria gonorrhoeae: topographical distribution of the outer membrane protein antigens. J Gen Microbiol. 1978 Oct;108(2):213–219. doi: 10.1099/00221287-108-2-213. [DOI] [PubMed] [Google Scholar]
  5. Loeb M. R., Zachary A. L., Smith D. H. Isolation and partial characterization of outer and inner membranes from encapsulated Haemophilus influenzae type b. J Bacteriol. 1981 Jan;145(1):596–604. doi: 10.1128/jb.145.1.596-604.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Manning P. A., Beutin L., Achtman M. Outer membrane of Escherichia coli: properties of the F sex factor traT protein which is involved in surface exclusion. J Bacteriol. 1980 Apr;142(1):285–294. doi: 10.1128/jb.142.1.285-294.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Markwell M. A., Fox C. F. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978 Oct 31;17(22):4807–4817. doi: 10.1021/bi00615a031. [DOI] [PubMed] [Google Scholar]
  8. Moriyon I., Berman D. T. Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope. J Bacteriol. 1982 Nov;152(2):822–828. doi: 10.1128/jb.152.2.822-828.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morrison M. Lactoperoxidase-catalyzed iodination as a tool for investigation of proteins. Methods Enzymol. 1980;70(A):214–220. doi: 10.1016/s0076-6879(80)70051-4. [DOI] [PubMed] [Google Scholar]
  10. Phillips D. R., Morrison M. Exposed protein on the intact human erythrocyte. Biochemistry. 1971 May 11;10(10):1766–1771. doi: 10.1021/bi00786a006. [DOI] [PubMed] [Google Scholar]
  11. Rodden J. L., Scocca J. J. Purification and properties of cyclic phosphodiesterase: 3'-nucleotidase, a periplasmic enzyme of Haemophilus influenzae. Arch Biochem Biophys. 1972 Dec;153(2):837–844. doi: 10.1016/0003-9861(72)90406-7. [DOI] [PubMed] [Google Scholar]
  12. Sullivan K. H., Williams R. P. Use of iodo-gen and iodine-125 to label the outer membrane proteins of whole cells of Neisseria gonorrhoeae. Anal Biochem. 1982 Mar 1;120(2):254–258. doi: 10.1016/0003-2697(82)90344-x. [DOI] [PubMed] [Google Scholar]
  13. Teather R. M., Müller-Hill B., Abrutsch U., Aichele G., Overath P. Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector. Mol Gen Genet. 1978 Feb 27;159(3):239–248. doi: 10.1007/BF00268260. [DOI] [PubMed] [Google Scholar]
  14. Zoon K. C., Scocca J. J. Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation. J Bacteriol. 1975 Aug;123(2):666–677. doi: 10.1128/jb.123.2.666-677.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES