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. 1983 Aug;155(2):643–649. doi: 10.1128/jb.155.2.643-649.1983

Mapping of the gene specifying aminoglycoside 3'-phosphotransferase II on the Pseudomonas aeruginosa chromosome.

M Okii, S Iyobe, S Mitsuhashi
PMCID: PMC217734  PMID: 6307974

Abstract

We examined the aminoglycoside inactivation enzymes in Pseudomonas aeruginosa strains, seven clinical isolates and seven laboratory strains without plasmids. All strains were found to possess the enzyme aminoglycoside 3'-phosphotransferase II [APH(3')-II]. We isolated an APH(3')-II-deficient mutant from a PAO strain by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. By plasmid (FP5 or R68.45)-mediated conjugation, we determined the locus of the gene specifying the APH(3')-II between trp-6 and pro-82 on the PAO chromosome and designated this gene aphA. It was concluded that the intrinsic resistance of P. aeruginosa to kanamycins, neomycins, paromomycins, ribostamycin, and butirosins was due to this newly determined gene.

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Selected References

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  1. Biddlecome S., Haas M., Davies J., Miller G. H., Rane D. F., Daniels P. J. Enzymatic modification of aminoglycoside antibiotics: a new 3-N-acetylating enzyme from a Pseudomonas aeruginosa isolate. Antimicrob Agents Chemother. 1976 Jun;9(6):951–955. doi: 10.1128/aac.9.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryan L. E., Nicas T., Holloway B. W., Crowther C. Aminoglycoside-resistant mutation of Pseudomonas aeruginosa defective in cytochrome c552 and nitrate reductase. Antimicrob Agents Chemother. 1980 Jan;17(1):71–79. doi: 10.1128/aac.17.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryan L. E., Van Den Elzen H. M., Tseng J. T. Transferable drug resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1972 Jan;1(1):22–29. doi: 10.1128/aac.1.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brzezinska M., Davies J. Two enzymes which phosphorylate neomycin and kanamycin in Escherichia coli strains carrying R factors. Antimicrob Agents Chemother. 1973 Feb;3(2):266–269. doi: 10.1128/aac.3.2.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies J., Smith D. I. Plasmid-determined resistance to antimicrobial agents. Annu Rev Microbiol. 1978;32:469–518. doi: 10.1146/annurev.mi.32.100178.002345. [DOI] [PubMed] [Google Scholar]
  6. Gray G. L., Vasil M. L. Isolation and genetic characterization of toxin-deficient mutants of Pseudomonas aeruginosa PAO. J Bacteriol. 1981 Aug;147(2):275–281. doi: 10.1128/jb.147.2.275-281.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  8. Holloway B. W. Isolation and characterization of an R' plasmid in Pseudomonas aeruginosa. J Bacteriol. 1978 Mar;133(3):1078–1082. doi: 10.1128/jb.133.3.1078-1082.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Iyobe S., Hasuda K., Fuse A., Mitsuhashi S. Demonstration of R factors from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Jun;5(6):547–552. doi: 10.1128/aac.5.6.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iyobe S., Sagai H., Mitsuhashi S. Tn2001, a transposon encoding chloramphenicol resistance in Pseudomonas aeruginosa. J Bacteriol. 1981 Apr;146(1):141–148. doi: 10.1128/jb.146.1.141-148.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. John J. F., Jr, McNeill W. F., Price K. E., Kresel P. A. Evidence for a chromosomal site specifying amikacin resistance in multiresistant Serratia marcescens. Antimicrob Agents Chemother. 1982 Apr;21(4):587–591. doi: 10.1128/aac.21.4.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kato T., Sato Y., Iyobe S., Mitsuhashi S. Plasmid-mediated gentamicin resistance of Pseudomonas aeruginosa and its lack of expression in Escherichia coli. Antimicrob Agents Chemother. 1982 Sep;22(3):358–363. doi: 10.1128/aac.22.3.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kawabe H., Inoue M., Mitsuhashi S. Inactivation of dihydrostreptomycin and spectinomycin by Staphylococcus aureus. Antimicrob Agents Chemother. 1974 Jun;5(6):553–557. doi: 10.1128/aac.5.6.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kawabe H., Naito T., Mitsuhashi S. Acetylation of amikacin, a new semisynthetic antibiotic, by Pseudomonas aeruginosa carrying an R factor. Antimicrob Agents Chemother. 1975 Jan;7(1):50–54. doi: 10.1128/aac.7.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kawabe H., Sakurai H., Fukasawa K., Shimizu S., Hasuda K., Iyobe S., Mitsuhashi S. Phosphorylation and inactivation of streptomycin by plant pathogenic Pseudomonas lachrymans. J Antibiot (Tokyo) 1979 Apr;32(4):425–426. doi: 10.7164/antibiotics.32.425. [DOI] [PubMed] [Google Scholar]
  16. Kayser F. H., Homberger F., Devaud M. Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus. Antimicrob Agents Chemother. 1981 May;19(5):766–772. doi: 10.1128/aac.19.5.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kondo S., Okanishi M., Utahara R., Maeda K., Umezawa H. Isolation of kanamycin and paromamine inactivated by E. coli carrying R factor. J Antibiot (Tokyo) 1968 Jan;21(1):22–29. doi: 10.7164/antibiotics.21.22. [DOI] [PubMed] [Google Scholar]
  18. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Matsuhashi Y., Sawa T., Takeuchi T., Umezawa H. Immunological studies of aminoglycoside 3'-phosphotransferases. J Antibiot (Tokyo) 1976 Oct;29(10):1127–1128. doi: 10.7164/antibiotics.29.1127. [DOI] [PubMed] [Google Scholar]
  21. Matsuhashi Y., Yagisawa M., Kondo S., Takeuchi T., Umezawa H. Aminoglycoside 3'-phosphotransferases I and II in Pseudomonas aeruginosa. J Antibiot (Tokyo) 1975 Jun;28(6):442–447. doi: 10.7164/antibiotics.28.442. [DOI] [PubMed] [Google Scholar]
  22. Matsumoto H., Ohta S., Kobayashi R., Terawaki Y. Chromosomal location of genes participating in the degradation of purines in Pseudomonas aeruginosa. Mol Gen Genet. 1978 Nov 29;167(2):165–176. doi: 10.1007/BF00266910. [DOI] [PubMed] [Google Scholar]
  23. Matsumoto H., Tazaki T. FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Sep;17(5):409–417. doi: 10.1111/j.1348-0421.1973.tb00792.x. [DOI] [PubMed] [Google Scholar]
  24. Mills B. J., Holloway B. W. Mutants of Pseudomonas aeruginosa that show specific hypersensitivity to aminoglycosides. Antimicrob Agents Chemother. 1976 Sep;10(3):411–416. doi: 10.1128/aac.10.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ozanne B., Benveniste R., Tipper D., Davies J. Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors. J Bacteriol. 1969 Nov;100(2):1144–1146. doi: 10.1128/jb.100.2.1144-1146.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Royle P. L., Matsumoto H., Holloway B. W. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1981 Jan;145(1):145–155. doi: 10.1128/jb.145.1.145-155.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sagai H., Krcmery V., Hasuda K., Iyobe S., Knothe H. R factor-mediated resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa. Jpn J Microbiol. 1975 Dec;19(6):427–432. doi: 10.1111/j.1348-0421.1975.tb00958.x. [DOI] [PubMed] [Google Scholar]
  28. Umezawa H. Biochemical mechanism of resistance to aminoglycosidic antibiotics. Adv Carbohydr Chem Biochem. 1974;30:183–225. doi: 10.1016/s0065-2318(08)60265-6. [DOI] [PubMed] [Google Scholar]
  29. Waltho J. A. Genetic analysis of phenylalanine-responding mutants of Pseudomonas aeruginosa. J Bacteriol. 1972 Dec;112(3):1070–1075. doi: 10.1128/jb.112.3.1070-1075.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yagisawa M., Naganawa H., Kondo S., Hamada M., Takeuchi T. Adenylyldideoxykanamycin B, a product of the inactivation of dideoxykanamycin B by Escherichia coli carrying R factor. J Antibiot (Tokyo) 1971 Dec;24(12):911–912. doi: 10.7164/antibiotics.24.911. [DOI] [PubMed] [Google Scholar]
  31. Yagisawa M., Yamamoto H., Naganawa H., Kondo S., Takeuchi T. A new enzyme in Escherichia coli carrying R-factor phosphorylating 3'-hydroxyl of butirosin A, kanamycin, neamine and ribostamycin. J Antibiot (Tokyo) 1972 Dec;25(12):748–750. doi: 10.7164/antibiotics.25.748. [DOI] [PubMed] [Google Scholar]

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