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. 1983 Aug;155(2):722–727. doi: 10.1128/jb.155.2.722-727.1983

Detailed restriction enzyme map of crown gall-suppressive IncW plasmid pSa, showing ends of deletion causing chloramphenicol sensitivity.

C R Ireland
PMCID: PMC217742  PMID: 6307978

Abstract

Detailed restriction enzyme maps of the antibiotic resistance plasmid pSa and of a chloramphenicol-sensitive spontaneous deletion mutant of pSa were constructed. The smaller plasmid contained one set of three restriction enzyme sites that appeared at both ends of the deleted region in the intact plasmid. The frequency of loss of chloramphenicol resistance was 1% after eight logs of growth and was dependent on the recA gene function of Escherichia coli. A previously published map lacks an 11-kilobase-pair SstII fragment present on this map.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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