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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Aug;155(2):755–760. doi: 10.1128/jb.155.2.755-760.1983

Amikacin resistance mediated by multiresistance transposon Tn2424.

J F Meyer, B A Nies, B Wiedemann
PMCID: PMC217747  PMID: 6307980

Abstract

Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.

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Selected References

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