Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Aug;155(2):903–906. doi: 10.1128/jb.155.2.903-906.1983

Extracellular suppression allows mating by pheromone-deficient sterile mutants of Saccharomyces cerevisiae.

R K Chan, L M Melnick, L C Blair, J Thorner
PMCID: PMC217767  PMID: 6348031

Abstract

MAT alpha haploids with mutations in the STE13 or KEX2 gene, and MATa haploids with mutations in the STE6 or STE14 gene, do not mate with wild-type cells of the opposite mating type. We found that such mutants were able to mate with partners that carry mutations (sst1 and sst2) that cause cells to be supersensitive to yeast mating pheromone action. Mating ability of MAT alpha ste13 and MAT alpha kex2 mutants could also be restored by adding normal MAT alpha cells to mating mixtures or by adding just the appropriate purified pheromone (alpha-factor). Therefore, the mating deficiencies caused by the ste13 and kex2 lesions, and by inference, the ste6 and ste14 mutations, appear to result only from secretion of an insufficient amount of pheromone or a nonfunctional pheromone.

Full text

PDF
903

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  2. Chan R. K., Otte C. A. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol. 1982 Jan;2(1):11–20. doi: 10.1128/mcb.2.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan R. K., Otte C. A. Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol. 1982 Jan;2(1):21–29. doi: 10.1128/mcb.2.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ciejek E., Thorner J. Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action. Cell. 1979 Nov;18(3):623–635. doi: 10.1016/0092-8674(79)90117-x. [DOI] [PubMed] [Google Scholar]
  5. Fehrenbacher G., Perry K., Thorner J. Cell-cell recognition in Saccharomyces cerevisiae: regulation of mating-specific adhesion. J Bacteriol. 1978 Jun;134(3):893–901. doi: 10.1128/jb.134.3.893-901.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GLEN W. L., BARBER R., MCCONKEY H. M., GRANT G. A. Isolation of beta-dihydroequilin and alpha-dihydroequilenin from the urine of pregnant mares. Nature. 1956 Apr 21;177(4512):753–753. doi: 10.1038/177753a0. [DOI] [PubMed] [Google Scholar]
  7. Hartwell L. H. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone. J Cell Biol. 1980 Jun;85(3):811–822. doi: 10.1083/jcb.85.3.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartwell L. H. Synchronization of haploid yeast cell cycles, a prelude to conjugation. Exp Cell Res. 1973 Jan;76(1):111–117. doi: 10.1016/0014-4827(73)90425-4. [DOI] [PubMed] [Google Scholar]
  9. Julius D., Blair L., Brake A., Sprague G., Thorner J. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Cell. 1983 Mar;32(3):839–852. doi: 10.1016/0092-8674(83)90070-3. [DOI] [PubMed] [Google Scholar]
  10. Leibowitz M. J., Wickner R. B. A chromosomal gene required for killer plasmid expression, mating, and spore maturation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2061–2065. doi: 10.1073/pnas.73.6.2061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mackay V., Manney T. R. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants. Genetics. 1974 Feb;76(2):255–271. doi: 10.1093/genetics/76.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackay V., Manney T. R. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. Genetics. 1974 Feb;76(2):273–288. doi: 10.1093/genetics/76.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Manney T. R., Woods V. Mutants of Saccharomyces cerevisiae resistant to the alpha mating-type factor. Genetics. 1976 Apr;82(4):639–644. doi: 10.1093/genetics/82.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rine J., Sprague G. F., Jr, Herskowitz I. rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation. Mol Cell Biol. 1981 Oct;1(10):958–960. doi: 10.1128/mcb.1.10.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sena E. P., Radin D. N., Fogel S. Synchronous mating in yeast. Proc Natl Acad Sci U S A. 1973 May;70(5):1373–1377. doi: 10.1073/pnas.70.5.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sprague G. F., Jr, Herskowitz I. Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1. J Mol Biol. 1981 Dec 5;153(2):305–321. doi: 10.1016/0022-2836(81)90280-1. [DOI] [PubMed] [Google Scholar]
  17. Sprague G. F., Jr, Rine J., Herskowitz I. Control of yeast cell type by the mating type locus. II. Genetic interactions between MAT alpha and unlinked alpha-specific STE genes. J Mol Biol. 1981 Dec 5;153(2):323–335. doi: 10.1016/0022-2836(81)90281-3. [DOI] [PubMed] [Google Scholar]
  18. Strathern J., Hicks J., Herskowitz I. Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis. J Mol Biol. 1981 Apr 15;147(3):357–372. doi: 10.1016/0022-2836(81)90488-5. [DOI] [PubMed] [Google Scholar]
  19. Wickner R. B. Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics. 1974 Mar;76(3):423–432. doi: 10.1093/genetics/76.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES