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. 1983 Aug;155(2):933–936. doi: 10.1128/jb.155.2.933-936.1983

DNA repair in competent cells of Bacillus subtilis.

I Mita, Y Sadaie, T Kada
PMCID: PMC217775  PMID: 6409889

Abstract

A series of isogenic transformable strains of Bacillus subtilis carrying the uvr-19 or rec-43 mutation or both were constructed. Both mutations made competent cells defective in repairing UV-irradiated cellular or transforming DNA, and their effects were additive in a doubly deficient strain, suggesting that two repair processes, requiring uvr-19+ and rec-43+ gene products, are independently functional in competent cells of B. subtilis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bron S., Venema G. Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives. Mutat Res. 1972 May;15(1):1–10. doi: 10.1016/0027-5107(72)90086-3. [DOI] [PubMed] [Google Scholar]
  2. Bron S., Venema G. Ultraviolet inactivation and excision-repair in Bacillus subtilis. II. Differential inactivation and differential repair of transforming markers. Mutat Res. 1972 May;15(1):11–22. doi: 10.1016/0027-5107(72)90087-5. [DOI] [PubMed] [Google Scholar]
  3. Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dodson L. A., Hadden C. T. Capacity for postreplication repair correlated with transducibility in Rec- mutants of Bacillus subtilis. J Bacteriol. 1980 Nov;144(2):608–615. doi: 10.1128/jb.144.2.608-615.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dodson L. A., Hadden C. T. Postreplication repair of deoxyribonucleic acid and daughter strand exchange in uvr- mutants of Bacillus subtilis. J Bacteriol. 1980 Nov;144(2):840–843. doi: 10.1128/jb.144.2.840-843.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dooley D. C., Hadden C. T., Nester E. W. Macromolecular synthesis in Bacillus subtilis during development of the competent state. J Bacteriol. 1971 Nov;108(2):668–679. doi: 10.1128/jb.108.2.668-679.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dubnau D., Cirigliano C. Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: nonrequirement of deoxyribonucleic acid replication for uptake and integration of transforming deoxyribonucleic acid. J Bacteriol. 1973 Mar;113(3):1512–1514. doi: 10.1128/jb.113.3.1512-1514.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dubnau D., Cirigliano C. Genetic characterization of recombination-deficient mutants of Bacillus subtilis. J Bacteriol. 1974 Feb;117(2):488–493. doi: 10.1128/jb.117.2.488-493.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Erickson R. J., Copeland J. C. Structure and replication of chromosomes in competent cells of Bacillus subtilis. J Bacteriol. 1972 Mar;109(3):1075–1084. doi: 10.1128/jb.109.3.1075-1084.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hadden C. T. Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant. J Bacteriol. 1979 Jul;139(1):239–246. doi: 10.1128/jb.139.1.239-246.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hadden C. T. Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis. J Bacteriol. 1981 Jan;145(1):434–441. doi: 10.1128/jb.145.1.434-441.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hadden C. T. Pyrimidine dimer excision in a Bacillus subtilis Uvr- mutant. J Bacteriol. 1979 Jul;139(1):247–255. doi: 10.1128/jb.139.1.247-255.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hadden C., Nester E. W. Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol. 1968 Mar;95(3):876–885. doi: 10.1128/jb.95.3.876-885.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Howard-Flanders P. DNA repair. Annu Rev Biochem. 1968;37:175–200. doi: 10.1146/annurev.bi.37.070168.001135. [DOI] [PubMed] [Google Scholar]
  15. Munakata N., Ikeda Y. Inactivation of transforming DNA by ultraviolet irradiation: a study with ultraviot-sensitive mutants of Bacillus subtilis. Mutat Res. 1969 Mar-Apr;7(2):133–139. doi: 10.1016/0027-5107(69)90025-6. [DOI] [PubMed] [Google Scholar]
  16. NESTER E. W. PENICILLIN RESISTANCE OF COMPETENT CELLS IN DEOXYRIBONUCLEIC ACID TRANSFORMATION OF BACILLUS SUBTILIS. J Bacteriol. 1964 Apr;87:867–875. doi: 10.1128/jb.87.4.867-875.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NESTER E. W., STOCKER B. A. BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1963 Oct;86:785–796. doi: 10.1128/jb.86.4.785-796.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sadaie Y., Kada T. Formation of competent Bacillus subtilis cells. J Bacteriol. 1983 Feb;153(2):813–821. doi: 10.1128/jb.153.2.813-821.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sadaie Y., Kada T. Recombination-deficient mutants of Bacillus subtilis. J Bacteriol. 1976 Feb;125(2):489–500. doi: 10.1128/jb.125.2.489-500.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Singh R. N., Pitale M. P. Competence and deoxyribonucleic acid uptake in Bacillus subtilis. J Bacteriol. 1968 Mar;95(3):864–866. doi: 10.1128/jb.95.3.864-866.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yasbin R. E. DNA repair in Bacillus subtilis. II. Activation of the inducible system in competent bacteria. Mol Gen Genet. 1977 Jun 8;153(2):219–225. [PubMed] [Google Scholar]
  22. Yasbin R. E., Fernwalt J. D., Fields P. I. DNA repair in Bacillus subtilis: excision repair capacity of competent cells. J Bacteriol. 1979 Jan;137(1):391–396. doi: 10.1128/jb.137.1.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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