Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Sep;155(3):983–988. doi: 10.1128/jb.155.3.983-988.1983

Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculus.

L G Burman, J Raichler, J T Park
PMCID: PMC217789  PMID: 6350274

Abstract

High-resolution autoradiography of thin sections of Escherichia coli cells whose murein was pulse-labeled with [3H]diaminopimelic acid after a period of diaminopimelic acid deprivation indicated that elongation of the murein sacculus occurs by a multisite (diffuse) process. Upon chasing, radioactivity in polar murein was stable, whereas radioactivity in cylindrical murein was reduced, indicating that diffuse intercalation of new murein occurred during cell elongation. Elongation and septation were shown to be overlapping processes.

Full text

PDF
983

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd A., Holland I. B. Protein d, an iron-transport protein induced by filtration of cultures of Escherichia coli. FEBS Lett. 1977 Apr 1;76(1):20–24. doi: 10.1016/0014-5793(77)80112-9. [DOI] [PubMed] [Google Scholar]
  2. Burdett I. D., Murray R. G. Electron microscope study of septum formation in Escherichia coli strains B and B-r during synchronous growth. J Bacteriol. 1974 Sep;119(3):1039–1056. doi: 10.1128/jb.119.3.1039-1056.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdett I. D., Murray R. G. Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division. J Bacteriol. 1974 Jul;119(1):303–324. doi: 10.1128/jb.119.1.303-324.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caro L. Progress in high-resolution autoradiography. Prog Biophys Mol Biol. 1966;16:171–190. doi: 10.1016/0079-6107(66)90006-x. [DOI] [PubMed] [Google Scholar]
  5. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Higgins M. L., Shockman G. D. Model for cell wall growth of Streptococcus faecalis. J Bacteriol. 1970 Feb;101(2):643–648. doi: 10.1128/jb.101.2.643-648.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koch A. L., Higgins M. L., Doyle R. J. Surface tension-like forces determine bacterial shapes: Streptococcus faecium. J Gen Microbiol. 1981 Mar;123(1):151–161. doi: 10.1099/00221287-123-1-151. [DOI] [PubMed] [Google Scholar]
  8. Lin E. C., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli. J Bacteriol. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Macalister T. J., Macdonald B., Rothfield L. I. The periseptal annulus: An organelle associated with cell division in Gram-negative bacteria. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1372–1376. doi: 10.1073/pnas.80.5.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mauck J., Chan L., Glaser L., Williamson J. Mode of cell wall growth of Bacillus megaterium. J Bacteriol. 1972 Jan;109(1):373–378. doi: 10.1128/jb.109.1.373-378.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ryter A., Hirota Y., Schwarz U. Process of cellular division in Escherichia coli growth pattern of E. coli murein. J Mol Biol. 1973 Jun 25;78(1):185–195. doi: 10.1016/0022-2836(73)90437-3. [DOI] [PubMed] [Google Scholar]
  12. Satta G., Fontana R., Canepari P., Botta G. Peptidoglycan synthesis in cocci and rods of a pH-dependent, morphologically conditional mutant of Klebsiella pneumoniae. J Bacteriol. 1979 Feb;137(2):727–734. doi: 10.1128/jb.137.2.727-734.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
  14. Schwarz U., Ryter A., Rambach A., Hellio R., Hirota Y. Process of cellular division in Escherichia coli: differention of growth zones in the Sacculus. J Mol Biol. 1975 Nov 15;98(4):749–759. doi: 10.1016/s0022-2836(75)80008-8. [DOI] [PubMed] [Google Scholar]
  15. Staugaard P., van den Berg F. M., Woldringh C. L., Nanninga N. Localization of ampicillin-sensitive sites in Escherichia coli by electron microscopy. J Bacteriol. 1976 Sep;127(3):1376–1381. doi: 10.1128/jb.127.3.1376-1381.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Steed P., Murray R. G. The cell wall and cell division of gram-negative bacteria. Can J Microbiol. 1966 Apr;12(2):263–270. doi: 10.1139/m66-036. [DOI] [PubMed] [Google Scholar]
  17. VAN TUBERGEN R. P., SETLOW R. B. Quantitative radioautographic studies on exponentially growing cultures of Escherichia coli. The distribution of parental DNA, RNA, protein, and cell wall among progeny cells. Biophys J. 1961 Sep;1:589–625. doi: 10.1016/s0006-3495(61)86911-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  19. Verwer R. W., Nanninga N. Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli. J Bacteriol. 1980 Oct;144(1):327–336. doi: 10.1128/jb.144.1.327-336.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES