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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Sep;155(3):1116–1122. doi: 10.1128/jb.155.3.1116-1122.1983

Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII.

L D Vales, B A Rabin, J W Chase
PMCID: PMC217806  PMID: 6350262

Abstract

Strains of Escherichia coli containing reduced levels of exonuclease VII activity due to mutations in the xseB gene have been isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Seven mutants of independent origin deficient in exonuclease VII activity were obtained. Four of these contained defects in xseA, a locus which has been previously identified, and three others contained mutations in a gene distinct from xseA, which we have designated xseB. Genetic mapping studies place the xseB locus between proC and dnaZ. Exonuclease VII purified from KLC835 (xseA+ xseB3) is more heat labile than enzyme purified from the parent strain PA610 (xse+), showing that xseB is a structural gene for exonuclease VII. The isolation of lambda transducing phage carrying xseA is also described.

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Selected References

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