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. 1983 Sep;155(3):1130–1137. doi: 10.1128/jb.155.3.1130-1137.1983

S gene product: identification and membrane localization of a lysis control protein.

E Altman, R K Altman, J M Garrett, R J Grimaila, R Young
PMCID: PMC217808  PMID: 6224771

Abstract

The product of the bacteriophage S gene has been previously shown to be required for an essential step in triggering host cell lysis. By using two different protein labeling systems, maxicells and UV-irradiated infected cells, we identified the S gene product as an 8,500-molecular-weight polypeptide associated with the cell envelope. The apparent molecular weight is significantly less than the 11,500 predicted from the S gene sequence. We were unable to confirm two previous identifications of S gene products, an acidic 15,000-molecular-weight polypeptide found by two-dimensional gel electrophoresis of infected cells and a 5,500-molecular-weight polypeptide in purified phage particles.

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Selected References

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