Abstract
Neisseria gonorrhoeae 82409/55(pJD1) is postulated to possess six DNA sequence-specific cytosine methyltransferases and one DNA sequence-specific N6-adenine methyltransferase. From the DNA sequencing of the plasmid pJD1 (manuscript in preparation) by a modification of the Maxam and Gilbert chemical cleavage procedure, the cytosine methylation specificities were demonstrated. Five of these methylating enzymes and their respective specificities are M . NgoI (formula; see text) does not methylate the cytosine of its recognition sequence, in agreement with a detected adenine modification. A biological implication of these different DNA methylating activities is discussed.
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Selected References
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- Clanton D. J., Riggsby W. S., Miller R. V. NgoII, a restriction endonuclease from Neisseria gonorrhoeae. J Bacteriol. 1979 Mar;137(3):1299–1307. doi: 10.1128/jb.137.3.1299-1307.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clanton D. J., Woodward J. M., Miller R. V. Identification of a new sequence-specific endonuclease, NgoII, from Neisseria gonorrhoeae. J Bacteriol. 1978 Jul;135(1):270–273. doi: 10.1128/jb.135.1.270-273.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Günthert U., Storm K., Bald R. Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence. Eur J Biochem. 1978 Oct 16;90(3):581–583. doi: 10.1111/j.1432-1033.1978.tb12638.x. [DOI] [PubMed] [Google Scholar]
- Hattman S., Keister T., Gottehrer A. Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis. J Mol Biol. 1978 Oct 5;124(4):701–711. doi: 10.1016/0022-2836(78)90178-x. [DOI] [PubMed] [Google Scholar]
- Mann M. B., Smith H. O. Specificity of Hpa II and Hae III DNA methylases. Nucleic Acids Res. 1977 Dec;4(12):4211–4221. doi: 10.1093/nar/4.12.4211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Norlander L., Davies J. K., Hagblom P., Normark S. Deoxyribonucleic acid modifications and restriction endonuclease production in Neisseria gonorrhoeae. J Bacteriol. 1981 Feb;145(2):788–795. doi: 10.1128/jb.145.2.788-795.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norlander L., Davies J., Norqvist A., Normark S. Genetic basis for colonial variation in Neisseria gonorrhoeae. J Bacteriol. 1979 Jun;138(3):762–769. doi: 10.1128/jb.138.3.762-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohmori H., Tomizawa J. I., Maxam A. M. Detection of 5-methylcytosine in DNA sequences. Nucleic Acids Res. 1978 May;5(5):1479–1485. doi: 10.1093/nar/5.5.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Radman M., Villani G., Boiteux S., Kinsella A. R., Glickman B. W., Spadari S. Replicational fidelity: mechanisms of mutation avoidance and mutation fixation. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):937–946. doi: 10.1101/sqb.1979.043.01.103. [DOI] [PubMed] [Google Scholar]
- Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1982 Mar 11;10(5):r117–r144. doi: 10.1093/nar/10.5.1770. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yuan R. Structure and mechanism of multifunctional restriction endonucleases. Annu Rev Biochem. 1981;50:285–319. doi: 10.1146/annurev.bi.50.070181.001441. [DOI] [PubMed] [Google Scholar]