Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Sep;155(3):1333–1342. doi: 10.1128/jb.155.3.1333-1342.1983

Characterization of Tn2411 and Tn2410, two transposons derived from R-plasmid R1767 and related to Tn2603 and Tn21.

J Kratz, F Schmidt, B Wiedemann
PMCID: PMC217832  PMID: 6309748

Abstract

Two transposable elements, Tn2410 and Tn2411, were isolated from Salmonella typhimurium R-factor R1767. They have sizes of 18.5 and 18.0 kilobases, respectively. Tn2411 mediates resistance to streptomycin, sulfonamides, and mercury. In Tn2410, the streptomycin resistance gene was replaced by a gene coding for the production of the beta-lactamase OXA-2, which is responsible for ampicillin resistance. Physical and functional maps of both transposons were compared with those of Tn21, Tn4, and Tn2603. From these data it appeared that Tn21 could be an ancestral transposon from which Tn2411, Tn2410, Tn2603, and Tn4 were evolved by the addition or deletion of small DNA segments.

Full text

PDF
1333

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett P. M., Grinsted J., Richmond M. H. Transposition of TnA does not generate deletions. Mol Gen Genet. 1977 Jul 20;154(2):205–211. doi: 10.1007/BF00330839. [DOI] [PubMed] [Google Scholar]
  2. Blohm D., Goebel W. Restriction map of the antibiotic resistance plasmid R1drd-19 and its derivatives pKN102 (R1drd-19B2) and R1drd-16 for the enzymes BamHI, HindIII, EcoRI and SalI. Mol Gen Genet. 1978 Nov 29;167(2):119–127. doi: 10.1007/BF00266905. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. COETZEE J. N. TRANSDUCTION OF SWARMING IN PROTEUS MIRABILIS. J Gen Microbiol. 1963 Oct;33:1–7. doi: 10.1099/00221287-33-1-1. [DOI] [PubMed] [Google Scholar]
  5. Chandler M., Silver L., Lane D., Caro L. Properties of an autonomous r-determinant from R100.1. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1223–1231. doi: 10.1101/sqb.1979.043.01.139. [DOI] [PubMed] [Google Scholar]
  6. Clerget M., Chandler M., Caro L. The structure of R1drd19: a revised physical map of the plasmid. Mol Gen Genet. 1981;181(2):183–191. doi: 10.1007/BF00268425. [DOI] [PubMed] [Google Scholar]
  7. Iida S. A cointegrate of the bacteriophage P1 genome and the conjugative R plasmid R100. Plasmid. 1980 May;3(3):278–290. doi: 10.1016/0147-619x(80)90041-4. [DOI] [PubMed] [Google Scholar]
  8. Iida S., Arber W. Plaque forming specialized transducing phage P1: isolation of P1CmSmSu, a precursor of P1Cm. Mol Gen Genet. 1977 Jun 24;153(3):259–269. doi: 10.1007/BF00431591. [DOI] [PubMed] [Google Scholar]
  9. Katsu K., Inoue M., Mitsuhashi S. Transposition of the carbenicillin-hydrolyzing beta-lactamase gene. J Bacteriol. 1982 May;150(2):483–489. doi: 10.1128/jb.150.2.483-489.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
  11. Klein R. D., Selsing E., Wells R. D. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis. Plasmid. 1980 Jan;3(1):88–91. doi: 10.1016/s0147-619x(80)90037-2. [DOI] [PubMed] [Google Scholar]
  12. Kopecko D. J., Brevet J., Cohen S. N. Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol. 1976 Dec;108(2):333–360. doi: 10.1016/s0022-2836(76)80124-6. [DOI] [PubMed] [Google Scholar]
  13. Kupersztoch-Portnoy Y. M., Lovett M. A., Helinski D. R. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K. Biochemistry. 1974 Dec 31;13(27):5484–5490. doi: 10.1021/bi00724a005. [DOI] [PubMed] [Google Scholar]
  14. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  15. Medeiros A. A., Hedges R. W., Jacoby G. A. Spread of a "Pseudomonas-specific" beta-lactamase to plasmids of enterobacteria. J Bacteriol. 1982 Feb;149(2):700–707. doi: 10.1128/jb.149.2.700-707.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Meyer J. F., Nies B. A., Wiedemann B. Amikacin resistance mediated by multiresistance transposon Tn2424. J Bacteriol. 1983 Aug;155(2):755–760. doi: 10.1128/jb.155.2.755-760.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miki T., Easton A. M., Rownd R. H. Mapping of the resistance genes of the R plasmid NR1. Mol Gen Genet. 1978 Jan 17;158(3):217–224. doi: 10.1007/BF00267192. [DOI] [PubMed] [Google Scholar]
  18. Mise K., Arber W. Plaque-forming transducing bacteriophage P1 derivatives and their behaviour in lysogenic conditions. Virology. 1976 Jan;69(1):191–205. doi: 10.1016/0042-6822(76)90206-3. [DOI] [PubMed] [Google Scholar]
  19. Ohtsubo H., Ohmori H., Ohtsubo E. Nucleotide-sequence analysis of Tn3 (ap): implications for insertion and deletion. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1269–1277. doi: 10.1101/sqb.1979.043.01.144. [DOI] [PubMed] [Google Scholar]
  20. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  21. Rownd R., Nakaya R., Nakamura A. Molecular nature of the drug-resistance factors of the Enterobacteriaceae. J Mol Biol. 1966 Jun;17(2):376–393. doi: 10.1016/s0022-2836(66)80149-3. [DOI] [PubMed] [Google Scholar]
  22. Rubens C. E., McNeill W. F., Farrar W. E., Jr Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. J Bacteriol. 1979 Sep;139(3):877–882. doi: 10.1128/jb.139.3.877-882.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  24. Schmidt F., Van Treeck U., Wiedemann B. Multimerization and replication of plasmid pBP11. Plasmid. 1982 Sep;8(2):126–140. doi: 10.1016/0147-619x(82)90051-8. [DOI] [PubMed] [Google Scholar]
  25. Tanak N., Cramer J. H., Rownd R. H. EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol. 1976 Jul;127(1):619–636. doi: 10.1128/jb.127.1.619-636.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tanaka M., Harafuji H., Yamamoto T. A gene and its product required for transposition of resistance transposon Tn2603. J Bacteriol. 1982 Aug;151(2):723–728. doi: 10.1128/jb.151.2.723-728.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tanaka M., Yamamoto T., Sawai T. Evolution of complex resistance transposons from an ancestral mercury transposon. J Bacteriol. 1983 Mar;153(3):1432–1438. doi: 10.1128/jb.153.3.1432-1438.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Timmis K. N., Cabello F., Cohen S. N. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet. 1978 Jun 14;162(2):121–137. doi: 10.1007/BF00267869. [DOI] [PubMed] [Google Scholar]
  29. Wood W. B. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J Mol Biol. 1966 Mar;16(1):118–133. doi: 10.1016/s0022-2836(66)80267-x. [DOI] [PubMed] [Google Scholar]
  30. Yamamoto T., Tanaka M., Baba R., Yamagishi S. Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance. Mol Gen Genet. 1981;181(4):464–469. doi: 10.1007/BF00428737. [DOI] [PubMed] [Google Scholar]
  31. Yamamoto T., Tanaka M., Nohara C., Fukunaga Y., Yamagishi S. Transposition of the oxacillin-hydrolyzing penicillinase gene. J Bacteriol. 1981 Feb;145(2):808–813. doi: 10.1128/jb.145.2.808-813.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. de la Cruz F., Grinsted J. Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1. J Bacteriol. 1982 Jul;151(1):222–228. doi: 10.1128/jb.151.1.222-228.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES