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. 1983 Sep;155(3):1426–1428. doi: 10.1128/jb.155.3.1426-1428.1983

Isolation of rel mutants of Escherichia coli B/r.

R Little, S W Mou, H Bremer
PMCID: PMC217843  PMID: 6350269

Abstract

A method that relies on the biological effect of near-UV (340-nm) irradiation is described by which large numbers of independent rel mutants of Escherichia coli B/r may be rapidly isolated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cashel M., Gallant J. Two compounds implicated in the function of the RC gene of Escherichia coli. Nature. 1969 Mar 1;221(5183):838–841. doi: 10.1038/221838a0. [DOI] [PubMed] [Google Scholar]
  2. Friesen J. D., Fiil N. P., Parker J. M., Haseltine W. A. A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3465–3469. doi: 10.1073/pnas.71.9.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friesen J. D., Parker J., Watson R. J., Fill N. P., Pedersen S., Pedersen F. S. Isolation of a lambda transducing bacteriophage carrying the relA gene of Escherichia coli. J Bacteriol. 1976 Aug;127(2):917–922. doi: 10.1128/jb.127.2.917-922.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Haseltine W. A., Block R., Gilbert W., Weber K. MSI and MSII made on ribosome in idling step of protein synthesis. Nature. 1972 Aug 18;238(5364):381–384. doi: 10.1038/238381a0. [DOI] [PubMed] [Google Scholar]
  5. Haseltine W. A., Block R. Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes. Proc Natl Acad Sci U S A. 1973 May;70(5):1564–1568. doi: 10.1073/pnas.70.5.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heinemeyer E. A., Richter D. Mechanism of the in vitro breakdown of guanosine 5'-diphosphate 3'-diphosphate in Escherichia coli. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4180–4183. doi: 10.1073/pnas.75.9.4180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kawasaki T., Nakata T., Nose Y. Genetic mapping with a thiamine-requiring auxotroph of Escherichia coli K-12 defective in thiamine phosphate pyrophosphorylase. J Bacteriol. 1968 Apr;95(4):1483–1485. doi: 10.1128/jb.95.4.1483-1485.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lavallé R. Nouveaux mutants de régulation de la synthèse de l'ARN. Bull Soc Chim Biol (Paris) 1965;47(8):1567–1570. [PubMed] [Google Scholar]
  9. Little R., Bremer H. Quantitation of guanosine 5',3'-bisdiphosphate in extracts from bacterial cells by ion-pair reverse-phase high-performance liquid chromatography. Anal Biochem. 1982 Nov 1;126(2):381–388. doi: 10.1016/0003-2697(82)90531-0. [DOI] [PubMed] [Google Scholar]
  10. Pao C. C., Gallant J. A gene involved in the metabolic control of ppGpp synthesis. Mol Gen Genet. 1978 Jan 17;158(3):271–277. doi: 10.1007/BF00267198. [DOI] [PubMed] [Google Scholar]
  11. Ramabhadran T. V. Effects of near-ultraviolet and violet radiations (313-405 nm) on DNA, RNA, and protein synthesis in E. coli B/r: implications for growth delay. Photochem Photobiol. 1975 Sep-Oct;22(3-4):117–123. doi: 10.1111/j.1751-1097.1975.tb08822.x. [DOI] [PubMed] [Google Scholar]
  12. Ramabhadran T. V., Jagger J. Mechanism of growth delay induced in Escherichia coli by near ultraviolet radiation. Proc Natl Acad Sci U S A. 1976 Jan;73(1):59–63. doi: 10.1073/pnas.73.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ramabhadran T. V. Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation. J Bacteriol. 1976 Sep;127(3):1587–1589. doi: 10.1128/jb.127.3.1587-1589.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Richter D. In vitro degradation of guanosine 3',5'-bis(diphosphate) [ppGpp] by the spoT gene product [ppGppase] from auxotrophic strains of Escherichia coli: effects of various antibiotics and drugs. Arch Microbiol. 1980 Feb;124(2-3):229–232. doi: 10.1007/BF00427731. [DOI] [PubMed] [Google Scholar]
  15. Ryals J., Hsu R. Y., Lipsett M. N., Bremer H. Isolation of single-site Escherichia coli mutants deficient in thiamine and 4-thiouridine syntheses: identification of a nuvC mutant. J Bacteriol. 1982 Aug;151(2):899–904. doi: 10.1128/jb.151.2.899-904.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Somerville C. R., Ahmed A. Mutants of Escherichia coli defective in the degradation of guanosine 5'-triphosphate, 3'-diphosphate (pppGpp). Mol Gen Genet. 1979 Feb 1;169(3):315–323. doi: 10.1007/BF00382277. [DOI] [PubMed] [Google Scholar]
  18. Yaniv M., Favre A., Barrell B. G. Structure of transfer RNA. Evidence for interaction between two non-adjacent nucleotide residues in tRNA from Escherichia coli. Nature. 1969 Sep 27;223(5213):1331–1333. doi: 10.1038/2231331a0. [DOI] [PubMed] [Google Scholar]

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