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. 1983 Sep;155(3):1446–1449. doi: 10.1128/jb.155.3.1446-1449.1983

Evidence for gene sharing in the nitrate reduction systems of Pseudomonas aeruginosa.

M Goldflam, J J Rowe
PMCID: PMC217848  PMID: 6411693

Abstract

Pseudomonas aeruginosa mutants unable to assimilate or dissimilate nitrate were isolated. Transduction and reversion analyses of these mutants revealed that single genetic lesions are responsible for the double phenotypes. The mutants were divided into two classes based on the ability to utilize hypoxanthine. It can be concluded from this study that at least two genes are shared between the two nitrate reduction systems.

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Selected References

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  1. 't Riet J van, Stouthamer A. H., Planta R. J. Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes. J Bacteriol. 1968 Nov;96(5):1455–1464. doi: 10.1128/jb.96.5.1455-1464.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amy N. K., Rajagopalan K. V. Characterization of molybdenum cofactor from Escherichia coli. J Bacteriol. 1979 Oct;140(1):114–124. doi: 10.1128/jb.140.1.114-124.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bray R. C. The reactions and the structures of molybdenum centers in enzymes. Adv Enzymol Relat Areas Mol Biol. 1980;51:107–165. doi: 10.1002/9780470122969.ch3. [DOI] [PubMed] [Google Scholar]
  4. Cove D. J. Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev Camb Philos Soc. 1979 Aug;54(3):291–327. doi: 10.1111/j.1469-185x.1979.tb01014.x. [DOI] [PubMed] [Google Scholar]
  5. Enoch H. G., Lester R. L. Effects of molybdate, tungstate, and selenium compounds on formate dehydrogenase and other enzyme systems in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1032–1040. doi: 10.1128/jb.110.3.1032-1040.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enoch H. G., Lester R. L. The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234–1241. doi: 10.1016/s0006-291x(74)80416-x. [DOI] [PubMed] [Google Scholar]
  7. Garrett R. H., Nason A. Further purification and properties of Neurospora nitrate reductase. J Biol Chem. 1969 Jun 10;244(11):2870–2882. [PubMed] [Google Scholar]
  8. Glaser J. H., DeMoss J. A. Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet. 1972;116(1):1–10. doi: 10.1007/BF00334254. [DOI] [PubMed] [Google Scholar]
  9. HOLLOWAY B. W., EGAN J. B., MONK M. Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1960 Aug;38:321–329. doi: 10.1038/icb.1960.34. [DOI] [PubMed] [Google Scholar]
  10. Johnson J. L., Hainline B. E., Rajagopalan K. V. Characterization of the molybdenum cofactor of sulfite oxidase, xanthine, oxidase, and nitrate reductase. Identification of a pteridine as a structural component. J Biol Chem. 1980 Mar 10;255(5):1783–1786. [PubMed] [Google Scholar]
  11. Ketchum P. A., Cambier H. Y., Frazier W. A., 3rd, Madansky C. H., Nason A. In vitro assembly of Neurospora assimilatory nitrate reductase from protein subunits of a Neurospora mutant and the xanthine oxidizing or aldehyde oxidase systems of higher animals. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1016–1023. doi: 10.1073/pnas.66.3.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lester R. L., DeMoss J. A. Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli. J Bacteriol. 1971 Mar;105(3):1006–1014. doi: 10.1128/jb.105.3.1006-1014.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MacGregor C. H. Anaerobic cytochrome b1 in Escherichia coli: association with and regulation of nitrate reductase. J Bacteriol. 1975 Mar;121(3):1111–1116. doi: 10.1128/jb.121.3.1111-1116.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Minagawa N., Yoshimoto A. Purification and characterization of the assimilatory NADPH-nitrate reductase of Aspergillus nidulans. J Biochem. 1982 Mar;91(3):761–774. doi: 10.1093/oxfordjournals.jbchem.a133763. [DOI] [PubMed] [Google Scholar]
  15. NASON A., EVANS H. J. Triphosphopyridine nucleotide-nitrate reductase in Neurospora. J Biol Chem. 1953 Jun;202(2):655–673. [PubMed] [Google Scholar]
  16. Nason A., Antoine A. D., Ketchum P. A., Frazier W. A., 3rd, Lee D. K. Formation of assimilatory nitrate reductase by in vitro inter-cistronic complementation in Neurospora crassa. Proc Natl Acad Sci U S A. 1970 Jan;65(1):137–144. doi: 10.1073/pnas.65.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nason A., Lee K. Y., Pan S. S., Ketchum P. A., Lamberti A., DeVries J. Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3242–3246. doi: 10.1073/pnas.68.12.3242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pienkos P. T., Klevickis S., Brill W. J. In vitro activation of inactive nitrogenase component I with molybdate. J Bacteriol. 1981 Jan;145(1):248–256. doi: 10.1128/jb.145.1.248-256.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pienkos P. T., Shah V. K., Brill W. J. Molybdenum cofactors from molybdoenzymes and in vitro reconstitution of nitrogenase and nitrate reductase. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5468–5471. doi: 10.1073/pnas.74.12.5468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scott R. H., Sperl G. T., DeMoss J. A. In vitro incorporation of molybdate into demolybdoproteins in Escherichia coli. J Bacteriol. 1979 Feb;137(2):719–726. doi: 10.1128/jb.137.2.719-726.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sias S. R., Ingraham J. L. Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate. Arch Microbiol. 1979 Sep;122(3):263–270. doi: 10.1007/BF00411289. [DOI] [PubMed] [Google Scholar]
  22. Sias S. R., Stouthamer A. H., Ingraham J. L. The assimilatory and dissimilatory nitrate reductases of Pseudomonas aeruginosa are encoded by different genes. J Gen Microbiol. 1980 May;118(1):229–234. doi: 10.1099/00221287-118-1-229. [DOI] [PubMed] [Google Scholar]
  23. Solomonson L. P., Lorimer G. H., Hall R. L., Borchers R., Bailey J. L. Reduced nicotinamide adenine dinucleotide-nitrate reductase of Chlorella vulgaris. Purification, prosthetic groups, and molecular properties. J Biol Chem. 1975 Jun 10;250(11):4120–4127. [PubMed] [Google Scholar]
  24. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  25. Van Hartingsveldt J., Marinus M. G., Stouthamer A. H. Mutants of Pseudomonas aeruginosa bblocked in nitrate or nitrite dissimilation. Genetics. 1971 Apr;67(4):469–482. doi: 10.1093/genetics/67.4.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van Hartingsveldt J., Stouthamer A. H. Mapping and characerization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth. J Gen Microbiol. 1973 Jan;74(1):97–106. doi: 10.1099/00221287-74-1-97. [DOI] [PubMed] [Google Scholar]

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