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. 1983 Nov;156(2):516–523. doi: 10.1128/jb.156.2.516-523.1983

Reversal of 2-bromoethanesulfonate inhibition of methanogenesis in Methanosarcina sp.

M R Smith
PMCID: PMC217862  PMID: 6313605

Abstract

2-Bromoethanesulfonate (BES) inhibition of methanogenesis from methanol by resting-cell suspensions or cell extracts of Methanosarcina was reversed by coenzyme M. BES inhibition of methylcoenzyme M methylreductase activity in cell-free extracts was reversed by methylcoenzyme M but not by coenzyme M. Methanol/coenzyme M methyltransferase activity was not inhibited by 10 microM BES. Inhibition of methylreductase by BES and 3-bromopropionate was competitive with methylcoenzyme M, but inhibition by 2-bromoethanol exhibited mixed kinetics. The Ki values for the inhibitors in cell-free extracts were similar to the concentrations which inhibited intact cells. BES-resistant mutants of strain 227 were apparently permeability mutants because in vitro assays showed that mutant and parent strain methylreductases were equally sensitive to BES.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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