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. 1983 Nov;156(2):686–694. doi: 10.1128/jb.156.2.686-694.1983

Transcriptional regulation of several genes for bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata in response to oxygen.

A J Biel, B L Marrs
PMCID: PMC217884  PMID: 6415036

Abstract

Although it has been shown that bacteriochlorophyll synthesis in Rhodopseudomonas capsulata is repressed by oxygen and high light intensity, few details of regulation by these environmental factors are known, primarily owing to a lack of assays for the biosynthetic enzymes. We have examined regulation at the transcriptional level by isolating and studying fusions between the Mu d1(Apr lac) phage and various bch genes. In these strains, the lacZ gene of the phage is under the control of bch gene promoters. We have found that atmospheric oxygen tension (20% O2) reduces the expression of these fusions at least twofold compared with low oxygen tension (2% O2). Therefore, transcription of the bchA, bchB, bchC, bchG, and bchH genes is regulated in response to oxygen.

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Selected References

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  1. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barth P. T. Plasmid RP4, with Escherichia coli DNA inserted in vitro, mediates chromosomal transfer. Plasmid. 1979 Jan;2(1):130–136. doi: 10.1016/0147-619x(79)90011-8. [DOI] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  6. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorchein A. Control of magnesium-protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Role of light, oxygen, and electron and energy transfer. Biochem J. 1973 Aug;134(4):833–845. doi: 10.1042/bj1340833d. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorchein A. Magnesium protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Studies with whole cells. Biochem J. 1972 Mar;127(1):97–106. doi: 10.1042/bj1270097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lascelles J. The accumulation of bacteriochlorophyll precursors by mutant and wild-type strains of Rhodopseudomonas spheroides. Biochem J. 1966 Jul;100(1):175–183. doi: 10.1042/bj1000175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lascelles J. The regulation of heme and chlorophyll synthesis in bacteria. Ann N Y Acad Sci. 1975 Apr 15;244:334–347. doi: 10.1111/j.1749-6632.1975.tb41540.x. [DOI] [PubMed] [Google Scholar]
  11. Marrs B. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid. J Bacteriol. 1981 Jun;146(3):1003–1012. doi: 10.1128/jb.146.3.1003-1012.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mindich L., Cohen J., Weisburd M. Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol. 1976 Apr;126(1):177–182. doi: 10.1128/jb.126.1.177-182.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nano F. E., Kaplan S. Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides. J Bacteriol. 1982 Nov;152(2):924–927. doi: 10.1128/jb.152.2.924-927.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pudek M. R., Richards W. R. A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides. Biochemistry. 1975 Jul 15;14(14):3132–3137. doi: 10.1021/bi00685a015. [DOI] [PubMed] [Google Scholar]
  15. Richards W. R., Lascelles J. The biosynthesis of bacteriochlorophyll. The characterization of latter stage intermediates from mutants of Rhodopseudomonas spheroides. Biochemistry. 1969 Aug;8(8):3473–3482. doi: 10.1021/bi00836a051. [DOI] [PubMed] [Google Scholar]
  16. Taylor D. P., Cohen S. N., Clark W. G., Marrs B. L. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol. 1983 May;154(2):580–590. doi: 10.1128/jb.154.2.580-590.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Watson M. D., Wild J., Umbarger H. E. Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY. J Bacteriol. 1979 Sep;139(3):1014–1020. doi: 10.1128/jb.139.3.1014-1020.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weaver P. F., Wall J. D., Gest H. Characterization of Rhodopseudomonas capsulata. Arch Microbiol. 1975 Nov 7;105(3):207–216. doi: 10.1007/BF00447139. [DOI] [PubMed] [Google Scholar]
  19. Yen H. C., Marrs B. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata. J Bacteriol. 1976 May;126(2):619–629. doi: 10.1128/jb.126.2.619-629.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Youvan D. C., Elder J. T., Sandlin D. E., Zsebo K., Alder D. P., Panopoulos N. J., Marrs B. L., Hearst J. E. R-prime site-directed transposon Tn7 mutagenesis of the photosynthetic apparatus in Rhodopseudomonas capsulata. J Mol Biol. 1982 Nov 25;162(1):17–41. doi: 10.1016/0022-2836(82)90160-7. [DOI] [PubMed] [Google Scholar]

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