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. 1983 Nov;156(2):904–906. doi: 10.1128/jb.156.2.904-906.1983

Escherichia coli xthA mutants are sensitive to inactivation by broad-spectrum near-UV (300- to 400-nm) radiation.

L J Sammartano, R W Tuveson
PMCID: PMC217911  PMID: 6355066

Abstract

Logarithmically growing and stationary-phase cells of Escherichia coli mutants lacking exonuclease III (xthA) were sensitive to inactivation by broad-spectrum near-UV (300- to 400-nm) radiation. The same xthA mutants were no more sensitive to far-UV wavelengths (200- to 300-nm) than was a strain bearing a functional xthA allele.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Demple B., Halbrook J., Linn S. Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. J Bacteriol. 1983 Feb;153(2):1079–1082. doi: 10.1128/jb.153.2.1079-1082.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dewitt S K, Adelberg E A. The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics. 1962 May;47(5):577–585. doi: 10.1093/genetics/47.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eisenstark A. Mutagenic and lethal effects of visible and near-ultraviolet light on bacterial cells. Adv Genet. 1971;16:167–198. [PubMed] [Google Scholar]
  4. Giloni L., Takeshita M., Johnson F., Iden C., Grollman A. P. Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage. J Biol Chem. 1981 Aug 25;256(16):8608–8615. [PubMed] [Google Scholar]
  5. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jagger J. Near-UV radiation effects on microorganisms. Photochem Photobiol. 1981 Dec;34(6):761–768. [PubMed] [Google Scholar]
  7. Klamen D. L., Tuveson R. W. The effect of membrane fatty acid composition on the near-UV (300-400 nm) sensitivity of Escherichia coli K1060. Photochem Photobiol. 1982 Feb;35(2):167–173. doi: 10.1111/j.1751-1097.1982.tb03827.x. [DOI] [PubMed] [Google Scholar]
  8. Milcarek C., Weiss B. Mutants of Escherichia coli with altered deoxyribonucleases. I. Isolation and characterization of mutants for exonuclease 3. J Mol Biol. 1972 Jul 21;68(2):303–318. doi: 10.1016/0022-2836(72)90215-x. [DOI] [PubMed] [Google Scholar]
  9. Peak J. G., Foote C. S., Peak M. J. Protection by DABCO against inactivation of transforming DNA by near-ultraviolet light: action spectra and implications for involvement of singlet oxygen. Photochem Photobiol. 1981 Jul;34(1):45–49. doi: 10.1111/j.1751-1097.1981.tb08959.x. [DOI] [PubMed] [Google Scholar]
  10. Peak M. J., Peak J. G. Protection by glycerol against the biological actions of near-ultraviolet light. Radiat Res. 1980 Sep;83(3):553–558. [PubMed] [Google Scholar]
  11. Peak M. J., Peak J. G. Single-strand breaks induced in Bacillus subtilis DNA by ultraviolet light: action spectrum and properties. Photochem Photobiol. 1982 May;35(5):675–680. doi: 10.1111/j.1751-1097.1982.tb02628.x. [DOI] [PubMed] [Google Scholar]
  12. Peters J., Jagger J. Inducible repair of near-UV radiation lethal damage in E. coli. Nature. 1981 Jan 15;289(5794):194–195. doi: 10.1038/289194a0. [DOI] [PubMed] [Google Scholar]
  13. RICHARDSON C. C., KORNBERG A. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY. J Biol Chem. 1964 Jan;239:242–250. [PubMed] [Google Scholar]
  14. RICHARDSON C. C., LEHMAN I. R., KORNBERG A. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY. J Biol Chem. 1964 Jan;239:251–258. [PubMed] [Google Scholar]
  15. Rogers S. G., Weiss B. Exonuclease III of Escherichia coli K-12, an AP endonuclease. Methods Enzymol. 1980;65(1):201–211. doi: 10.1016/s0076-6879(80)65028-9. [DOI] [PubMed] [Google Scholar]
  16. Taylor A. F., Weiss B. Role of exonuclease III in the base excision repair of uracil-containing DNA. J Bacteriol. 1982 Jul;151(1):351–357. doi: 10.1128/jb.151.1.351-357.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tuveson R. W. Genetic control of near-UV sensitivity independent of excision deficiency (uvrA6) in Escherichia coli K12. Photochem Photobiol. 1980 Nov;32(5):703–705. doi: 10.1111/j.1751-1097.1980.tb04044.x. [DOI] [PubMed] [Google Scholar]
  18. Tuveson R. W., Jonas R. B. Genetic control of near-UV (300-400 NM) sensitivity independent of the recA gene in strains of Escherichia coli K12. Photochem Photobiol. 1979 Dec;30(6):667–676. doi: 10.1111/j.1751-1097.1979.tb07197.x. [DOI] [PubMed] [Google Scholar]
  19. Tuveson R. W., Peak J. G., Peak M. J. Single-strand DNA breaks induced by 365 NM radiation in Escherichia coli strains differing in sensitivity to near and far UV. Photochem Photobiol. 1983 Jan;37(1):109–112. doi: 10.1111/j.1751-1097.1983.tb04442.x. [DOI] [PubMed] [Google Scholar]
  20. Tuveson R. W. The interaction of a gene (nur) controlling near-UV sensitivity and the polA1 gene in strains of E. coli K12. Photochem Photobiol. 1981 Jun;33(6):919–923. doi: 10.1111/j.1751-1097.1981.tb05513.x. [DOI] [PubMed] [Google Scholar]
  21. Warner H. R., Demple B. F., Deutsch W. A., Kane C. M., Linn S. Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4602–4606. doi: 10.1073/pnas.77.8.4602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Webb R. B., Tuveson R. W. Differential sensitivity to inactivation of nur and nur+ strains of Escherichia coli at six selected wavelengths in the UVA, UVB and UVC ranges. Photochem Photobiol. 1982 Nov;36(5):525–530. doi: 10.1111/j.1751-1097.1982.tb04411.x. [DOI] [PubMed] [Google Scholar]
  23. Weiss B. Endonuclease II of Escherichia coli is exonuclease III. J Biol Chem. 1976 Apr 10;251(7):1896–1901. [PubMed] [Google Scholar]
  24. White B. J., Hochhauser S. J., Cintron N. M., Weiss B. Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12. J Bacteriol. 1976 Jun;126(3):1082–1088. doi: 10.1128/jb.126.3.1082-1088.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yajko D. M., Weiss B. Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Feb;72(2):688–692. doi: 10.1073/pnas.72.2.688. [DOI] [PMC free article] [PubMed] [Google Scholar]

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