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. 1983 Nov;156(2):970–974. doi: 10.1128/jb.156.2.970-974.1983

Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.

T A Baker, M M Howe, C A Gross
PMCID: PMC217929  PMID: 6226649

Abstract

We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties of Mu d1 but produces temperature-resistant lysogens and transposes at a reduced frequency. A method is described to convert existing Mu d1 insertions to Mu dX.

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Selected References

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