Abstract
The total (1 leads to 3)-beta-glucanase activities associated with cell extracts and cell walls of Saccharomyces cerevisiae were measured during vegetative growth, conjugation, and sporulation. Using a system of column chromatography, we resolved (1 leads to 3)-beta-glucanase activity into six different enzymes (namely, glucanases I, II, IIIA, IIIB, IV, and V). The contributions of the individual enzymes to the total activity at the different stages of the life cycle were determined. Total glucanase activity increased during exponential growth and decreased in stationary resting-phase cells. Glucanase IIIA was the predominant enzyme in stationary resting-phase cells. Glucanases I, II, IIIB, and IV were either absent or present at low levels in stationary phase cells, but their individual activities (in particular, glucanase IIIB activity) increased substantially during exponential growth. Total (1 leads to 3)-beta-glucanase activity did not change significantly during conjugation of two haploid mating strains, S. cerevisiae 2180A and 2180B, and no notable changes were detected in the activities of the individual enzymes. Sporulation was accompanied by a rapid increase and then a decrease in total glucanase activity. Most of the increase was due to a dramatic rise in the activity of glucanase V, which appeared to be a sporulation-specific enzyme. Glucanase activity was not derepressed by lowering the glucose concentration in the growth medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cortat M., Matile P., Wiemken A. Isolation of glucanase-containing vesicles from budding yeast. Arch Mikrobiol. 1972;82(3):189–205. doi: 10.1007/BF00412191. [DOI] [PubMed] [Google Scholar]
- Crandall M., Egel R., Mackay V. L. Physiology of mating in three yeasts. Adv Microb Physiol. 1977;15:307–398. doi: 10.1016/s0065-2911(08)60319-7. [DOI] [PubMed] [Google Scholar]
- Fleet G. H., Phaff H. J. Glucanases in Schizosaccharomyces. Isolation and properties of an exo-beta-glucanase from the cell extracts and culture fluid of Schizosaccharomyces japonicus var. versatilis. Biochim Biophys Acta. 1975 Dec 18;410(2):318–332. doi: 10.1016/0005-2744(75)90234-x. [DOI] [PubMed] [Google Scholar]
- Reichelt B. Y., Fleet G. H. Isolation, properties, function, and regulation of endo-(1 leads to 3)-beta-glucanases in Schizosaccharomyces pombe. J Bacteriol. 1981 Sep;147(3):1085–1094. doi: 10.1128/jb.147.3.1085-1094.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Santos T., del Rey F., Conde J., Villanueva J. R., Nombela C. Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production. J Bacteriol. 1979 Aug;139(2):333–338. doi: 10.1128/jb.139.2.333-338.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zonneveld B. J. Morphogenesis in Aspergillus nidulans. The significance of a alpha-1, 3-glucan of the cell wall and alpha-1, 3-glucanase for cleistothecium development. Biochim Biophys Acta. 1972 Jun 26;273(1):174–187. doi: 10.1016/0304-4165(72)90205-x. [DOI] [PubMed] [Google Scholar]
- del Rey F., Santos T., García-Acha I., Nombela C. Synthesis of 1,3-beta-glucanases in Saccharomyces cerevisiae during the mitotic cycle, mating, and sporulation. J Bacteriol. 1979 Sep;139(3):924–931. doi: 10.1128/jb.139.3.924-931.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- del Rey F., Santos T., García-Acha I., Nombela C. Synthesis of beta-glucanases during sporulation in Saccharomyces cerevisiae: formation of a new, sporulation-specific 1,3-beta-glucanase. J Bacteriol. 1980 Aug;143(2):621–627. doi: 10.1128/jb.143.2.621-627.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- del Rey F., Villa T. G., Santos T., Garcia-Acha I., Nombela C. Purification and partial characterization of a new, sporulation specific, exo-beta-glucanase from Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1982 Apr 29;105(4):1347–1353. doi: 10.1016/0006-291x(82)90935-4. [DOI] [PubMed] [Google Scholar]