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. 1983 Dec;156(3):1292–1300. doi: 10.1128/jb.156.3.1292-1300.1983

Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria.

G Selvaraj, V N Iyer
PMCID: PMC217980  PMID: 6315684

Abstract

We describe the construction and use of a set of plasmid vectors of the transposons Tn1, Tn5, and Tn9 that are suicidal in Rhizobium species and therefore suitable for mutagenesis with these three transposons. The vectors are composed of the p15A replicon which functions in Escherichia coli but not in Rhizobium species and a region encoding the N type of bacterial conjugation system which is very efficient in matings between E. coli and Rhizobium species. The usefulness of the vectors has been most extensively assessed in Rhizobium meliloti. It is likely that they will be useful for mutagenesis and genome manipulation in other bacteria as well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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