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. 1979 Sep;139(3):730–737. doi: 10.1128/jb.139.3.730-737.1979

Two Escherichia coli chromosomal cistrons, sfrA and sfrB, which are needed for expression of F factor tra functions.

L Beutin, M Achtman
PMCID: PMC218016  PMID: 383690

Abstract

Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sfrB mutations. sfrA and sfrB are cistrons mapping near thr and metE, respectively. Several F-like plasmids are dependent on sfrA and on sfrB for expression of tra cistrons. Plasmids of incompatibility groups C and S are only dependent on sfrB,and other conjugative plasmids are dependent on neither. sfrB mutations also result in changes in certain cell envelope properties, including change sensitivity to certain bacteriophages which use lipopolysaccharide as a receptor, synthesis of nonfunctional flagella, and altered sensitivity to antibiotics.

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Selected References

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  1. Achtman M. Mating aggregates in Escherichia coli conjugation. J Bacteriol. 1975 Aug;123(2):505–515. doi: 10.1128/jb.123.2.505-515.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Achtman M., Skurray R. A., Thompson R., Helmuth R., Hall S., Beutin L., Clark A. J. Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol. 1978 Mar;133(3):1383–1392. doi: 10.1128/jb.133.3.1383-1392.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Achtman M., Willetts N., Clark A. J. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Armstrong J. B., Adler J., Dahl M. M. Nonchemotactic mutants of Escherichia coli. J Bacteriol. 1967 Jan;93(1):390–398. doi: 10.1128/jb.93.1.390-398.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Boman H. G., Monner D. A. Characterization of lipopolysaccharides from Escherichia coli K-12 mutants. J Bacteriol. 1975 Feb;121(2):455–464. doi: 10.1128/jb.121.2.455-464.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chakrabarti S., Gorini L. Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli. J Bacteriol. 1975 Feb;121(2):670–674. doi: 10.1128/jb.121.2.670-674.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fan D. P. Deletions in limited homology recombination in Escherichia coli. Genetics. 1969 Feb;61(2):351–361. doi: 10.1093/genetics/61.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finnegan D. J., Willetts N. S. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Mol Gen Genet. 1971;111(3):256–264. doi: 10.1007/BF00433110. [DOI] [PubMed] [Google Scholar]
  11. Finnegan D., Willetts N. The nature of the transfer inhibitor of several F-like plasmids. Mol Gen Genet. 1972;119(1):57–66. doi: 10.1007/BF00270444. [DOI] [PubMed] [Google Scholar]
  12. Finnegan D., Willetts N. The site of action of the F transfer inhibitor. Mol Gen Genet. 1973 Dec 31;127(4):307–316. doi: 10.1007/BF00267101. [DOI] [PubMed] [Google Scholar]
  13. Hancock R. E., Reeves P. Bacteriophage resistance in Escherichia coli K-12: general pattern of resistance. J Bacteriol. 1975 Mar;121(3):983–993. doi: 10.1128/jb.121.3.983-993.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Helmuth R., Achtman M. Operon structure of DNA transfer cistrons on the F sex factor. Nature. 1975 Oct 23;257(5528):652–656. doi: 10.1038/257652a0. [DOI] [PubMed] [Google Scholar]
  15. Hoar D. I. Fertility regulation in F-like resistance transfer factors. J Bacteriol. 1970 Mar;101(3):916–920. doi: 10.1128/jb.101.3.916-920.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kalckar H. M., Laursen P., Rapin A. M. Inactivation of phage c21 by various preparations from lipopolysaccharide of e. Coli k-12. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1852–1858. doi: 10.1073/pnas.56.6.1852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lerner T. J., Zinder N. D. Chromosomal regulation of sexual expression in Escherichia coli. J Bacteriol. 1979 Feb;137(2):1063–1065. doi: 10.1128/jb.137.2.1063-1065.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rapin A. M., Kalckar H. M., Alberico L. The metabolic basis for masking of receptor-sites on E. coli K-12 for C21, a lipopolysaccharide core-specific phage. Arch Biochem Biophys. 1968 Oct;128(1):95–105. doi: 10.1016/0003-9861(68)90011-8. [DOI] [PubMed] [Google Scholar]
  19. Riva S., Fietta A. M., Silvestri L. G., Romero E. Effect of rifampicin on expression of some episomal genes in E. coli. Nat New Biol. 1972 Jan 19;235(55):78–80. doi: 10.1038/newbio235078a0. [DOI] [PubMed] [Google Scholar]
  20. Romero E., Riva S., Berti M., Fietta A. M., Silvestri L. G. Pleiotropic effects of a rifampicin-resistant mutation in E. coli. Nat New Biol. 1973 Dec 19;246(155):225–228. doi: 10.1038/newbio246225a0. [DOI] [PubMed] [Google Scholar]
  21. Sabourin D., Beckwith J. Deletion of the Escherichia coli crp gene. J Bacteriol. 1975 Apr;122(1):338–340. doi: 10.1128/jb.122.1.338-340.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schade S., Adler J. Purification and chemistry of bacteriophage chi. J Virol. 1967 Jun;1(3):591–598. doi: 10.1128/jvi.1.3.591-598.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watson G., Paigen K. Isolation and characterization of an Escherichia coli bacteriophage requiring cell wall galactose. J Virol. 1971 Nov;8(5):669–674. doi: 10.1128/jvi.8.5.669-674.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Willetts N. The transcriptional control of fertility in F-like plasmids. J Mol Biol. 1977 May 5;112(1):141–148. doi: 10.1016/s0022-2836(77)80161-7. [DOI] [PubMed] [Google Scholar]

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