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. 1979 Sep;139(3):842–849. doi: 10.1128/jb.139.3.842-849.1979

Ribosomal ribonucleic acid isolated from Salmonella typhimurium: absence of the intact 23S species.

M E Winkler
PMCID: PMC218030  PMID: 383696

Abstract

Ribonucleic acid (RNA) isolated by four distinct methods and from a variety of Salmonella typhimurium strains lacked intact 23S ribosomal RNA (rRNA). On sucrose gradients which minimize aggregation, the vast majority of S. typhimurium rRNA sedimented as a 16S peak with a 14S shoulder. RNA from this region of the gradient was resolved into three discrete bands by electrophoresis in formamide. Two very minor S. typhimurium RNA peaks were resolved at 21S and 10S on sucrose gradients, and each peak formed discrete bands in electrophoresis. It is concluded that if S. typhimurium does possess an intact 23S rRNA species, this species is extremely "labile." The absence of isolatable S. typhimurium 23S rRNA possibly reflected in vivo processing of the rRNA before isolation. Under certain conditions, S. typhimurium rRNA formed discrete aggregates which sedimented similarly to intact Escherichia coli 23S rRNA.

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Selected References

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  1. Antón D. N. Histidine regulatory mutants in Salmonella typhimurium. V. Two new classes histidine regulatory mutants. J Mol Biol. 1968 May 14;33(3):533–546. doi: 10.1016/0022-2836(68)90304-5. [DOI] [PubMed] [Google Scholar]
  2. Bossi L., Ciampi M. S., Cortese R. Characterization of a Salmonella typhimurium hisU mutant defective in tRNA precursor processing. J Bacteriol. 1978 May;134(2):612–620. doi: 10.1128/jb.134.2.612-620.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doolittle W. F. Postmaturational cleavage of 23s ribosomal ribonucleic acid and its metabolic control in the blue-green alga Anacystis nidulans. J Bacteriol. 1973 Mar;113(3):1256–1263. doi: 10.1128/jb.113.3.1256-1263.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ely B., Fankhauser D. B., Hartman P. E. A fine structure map of the salmonella histidine operator-promoter. Genetics. 1974 Oct;78(2):607–631. doi: 10.1093/genetics/78.2.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ennis H. L., Kievitt K. D. Beta-Galactosidase messenger RNA made during recovery from inhibition of protein synthesis is not translated. J Biol Chem. 1976 May 10;251(9):2854–2860. [PubMed] [Google Scholar]
  6. Forchhammer J., Jackson E. N., Yanofsky C. Different half-lives of messenger RNA corresponding to different segments of the tryptophan operon of Escherichia coli. J Mol Biol. 1972 Nov 28;71(3):687–699. doi: 10.1016/s0022-2836(72)80032-9. [DOI] [PubMed] [Google Scholar]
  7. Grienenberger J. M., Simon D. Etude de la structure et de la dégradadation des acides ribonucléiques ribosomaux de quelques bactéries du genre Agrobacterium. C R Acad Sci Hebd Seances Acad Sci D. 1972 Feb 28;274(9):1399–1402. [PubMed] [Google Scholar]
  8. Honjo T., Swan D., Packman S., Polsky F., Leder P. Purification and translation of an immunoglobulin lambda chain messenger RNA from mouse myeloma. Biochemistry. 1976 Jun 29;15(13):2775–2779. doi: 10.1021/bi00658a011. [DOI] [PubMed] [Google Scholar]
  9. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
  10. Korn L. J., Yanofsky C. Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli. J Mol Biol. 1976 May 15;103(2):395–409. doi: 10.1016/0022-2836(76)90319-3. [DOI] [PubMed] [Google Scholar]
  11. Lessie T. G. The atypical ribosomal RNA complement of Rhodopseudomonas spheroides. J Gen Microbiol. 1965 Jun;39(3):311–320. doi: 10.1099/00221287-39-3-311. [DOI] [PubMed] [Google Scholar]
  12. MIDGLEY J. E. EFFECTS OF DIFFERENT EXTRACTION PROCEDURES ON THE MOLECULAR CHARACTERISTICS OF BACTERIAL RIBOSOMAL RIBONUCLEIC ACID. Biochim Biophys Acta. 1965 Feb 8;95:232–243. doi: 10.1016/0005-2787(65)90488-0. [DOI] [PubMed] [Google Scholar]
  13. Marrs B., Kaplan S. 23 s precursor ribosomal RNA of Rhodopseudomonas spheroides. J Mol Biol. 1970 Apr 28;49(2):297–317. doi: 10.1016/0022-2836(70)90247-0. [DOI] [PubMed] [Google Scholar]
  14. Midgley J. E. Studies on the structure of 23-S ribosomal ribonucleic acid from Escherichia coli. Biochim Biophys Acta. 1965 Nov 8;108(3):348–354. doi: 10.1016/0005-2787(65)90027-4. [DOI] [PubMed] [Google Scholar]
  15. Pace N. R. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriol Rev. 1973 Dec;37(4):562–603. doi: 10.1128/br.37.4.562-603.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  17. Robinson A., Sykes J. A study of the atypical ribosomal RNA components of Rhodopseudomonas spheroides. Biochim Biophys Acta. 1971 Apr 29;238(1):99–115. doi: 10.1016/0005-2787(71)90014-1. [DOI] [PubMed] [Google Scholar]
  18. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Summers W. C. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate. Anal Biochem. 1970 Feb;33(2):459–463. doi: 10.1016/0003-2697(70)90316-7. [DOI] [PubMed] [Google Scholar]
  20. Suryanarayana T., Burma D. P. Substrate specificity of Salmonella typhimurium RNAase III and the nature of products formed. Biochim Biophys Acta. 1975 Nov 4;407(4):459–468. doi: 10.1016/0005-2787(75)90299-3. [DOI] [PubMed] [Google Scholar]
  21. Sypherd P. S., O'Neil D. M., Taylor M. M. The chemical and genetic structure of bacterial ribosomes. Cold Spring Harb Symp Quant Biol. 1969;34:77–84. doi: 10.1101/sqb.1969.034.01.012. [DOI] [PubMed] [Google Scholar]
  22. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  23. Venetianer P., Berberich M. A., Goldberger R. F. Studies on the size of the messenger-RNA transcribed from the histidine operon during simultaneous and sequential depression. Biochim Biophys Acta. 1968 Aug 23;166(1):124–133. doi: 10.1016/0005-2787(68)90496-6. [DOI] [PubMed] [Google Scholar]
  24. Wehr C. T. Isolation and properties of a ribonuclease-deficient mutant of Salmonella typhimurium. J Bacteriol. 1973 Apr;114(1):96–102. doi: 10.1128/jb.114.1.96-102.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ZINDER N. D., LEDERBERG J. Genetic exchange in Salmonella. J Bacteriol. 1952 Nov;64(5):679–699. doi: 10.1128/jb.64.5.679-699.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]

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