Abstract
The growth characteristics of influenza virus in an isolated tissue maintained in vitro have been described. When compared with previously reported results using the embryonate egg, a considerably shorter latent period was observed. The release or liberation of the virus occurred throughout a period of many hours. There was no evidence of a general "burst" phenomenon, and the destruction of a cellular membrane did not seem to be essential to or concomitant with the release of virus. An early phase in the development of virus was described which is sensitive to the action of α-amino-p-methoxyphenylmethanesulfonic acid and it is by virtue of this that virus multiplication is prevented. If this phase was allowed to go on to completion, replication of virus occurred even in the presence of the sulfonic acid, but the release of virus from the tissue was impaired. It is suggested that the sulfonic acid may interfere with the adsorption or penetration of the virus and that the initiation of infection and the liberation of new virus may be processes which share some common character.
Full Text
The Full Text of this article is available as a PDF (642.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ACKERMANN W. W. Alpha-Aminosulfonic acids and viral propagation. Proc Soc Exp Biol Med. 1952 Jun;80(2):362–367. doi: 10.3181/00379727-80-19624. [DOI] [PubMed] [Google Scholar]
- ACKERMANN W. W., JOHNSON R. B. Some energy relations in a host-virus system. J Exp Med. 1953 Mar;97(3):315–322. doi: 10.1084/jem.97.3.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ACKERMANN W. W., KLERNSCHMIDT E. Concerning the relation of the Krebs cycle to virus propagation. J Biol Chem. 1951 Mar;189(1):421–428. [PubMed] [Google Scholar]
- ACKERMANN W. W., KURTZ H. The relation of herpes virus to host cell mitochondria. J Exp Med. 1952 Aug;96(2):151–157. doi: 10.1084/jem.96.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENLE W. Studies on host-virus interactions in the chick embryo-influenza virus system; the propagation of virus in conjunction with the host cells. J Exp Med. 1949 Jul;90(1):13–22. doi: 10.1084/jem.90.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henle W., Henle G., Rosenberg E. B. THE DEMONSTRATION OF ONE-STEP GROWTH CURVES OF INFLUENZA VIRUSES THROUGH THE BLOCKING EFFECT OF IRRADIATED VIRUS ON FURTHER INFECTION. J Exp Med. 1947 Oct 31;86(5):423–437. doi: 10.1084/jem.86.5.423. [DOI] [PMC free article] [PubMed] [Google Scholar]