Abstract
Contact of a purified endotoxin from Shigella fiexneri Type Z with normal rabbit or human serum results in an alteration of its immunological reaction with antiserum, as determined by precipitation in gel. Analysis of fractions of normal serum obtained by zone electrophoresis in starch indicates that the component responsible for altering the immunological reaction of endotoxin is associated with beta globulin. Normal serum has no similar effect on the immunological reaction of a variety of other protein and polysaccharide antigens. Serum from rabbits made tolerant to the pyrogenic action of an endotoxin from Serratia marcescens (P-35) possesses the ability to alter the reaction of Shigella endotoxin with its specific antiserum, although the serum from tolerant rabbits does not significantly enhance the pyrogenicity of Shigella toxm. The component of normal rabbit serum responsible for the effect on the immunological reaction of Shigella endotoxin is not destroyed by heating at 56°C. The possible relationship of the effect of normal serum on the immunological reaction of endotoxin and the augmentation of fever induced by endotoxins is discussed.
Full Text
The Full Text of this article is available as a PDF (760.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLUFF L. E. Immunochemical study of a bacterial endotoxin: Shigella flexneri type Z. J Exp Med. 1954 Oct 1;100(4):391–404. doi: 10.1084/jem.100.4.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FARR R. S., LeQUIRE V. S. Leukocytic and pyrogenic effects of typhoid vaccine and augmentation by homologous plasma. Proc Soc Exp Biol Med. 1950 Dec;75(3):661–666. doi: 10.3181/00379727-75-18298. [DOI] [PubMed] [Google Scholar]
- GRANT R. Refractorines to pyrogens, effects of incubation of pyrogen with plasma from normal and refractory donors on the responses of refractory recipients. Am J Physiol. 1953 May;173(2):246–252. doi: 10.1152/ajplegacy.1953.173.2.246. [DOI] [PubMed] [Google Scholar]
- GRANT R., WHALEN W. J. Latency of pyrogen fever; appearance of a fast-acting pyrogen in the blood of febrile animals and in plasma incubated with bacterial pyrogen. Am J Physiol. 1953 Apr;173(1):47–54. doi: 10.1152/ajplegacy.1953.173.1.47. [DOI] [PubMed] [Google Scholar]
- KUNKEL H. G., SLATER R. J. Zone electrophoresis in a starch supporting medium. Proc Soc Exp Biol Med. 1952 May;80(1):42–44. doi: 10.3181/00379727-80-19516. [DOI] [PubMed] [Google Scholar]
- KUNKEL H. G., TISELIUS A. Electrophoresis of proteins on filter paper. J Gen Physiol. 1951 Sep;35(1):89–118. doi: 10.1085/jgp.35.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan W. T., Partridge S. M. Studies in immunochemistry: The use of phenol and of alkali in the degradation of antigenic material isolated from Bact. dysenteriae (Shiga). Biochem J. 1941 Nov;35(10-11):1140–1163. doi: 10.1042/bj0351140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan W. T. Studies in immuno-chemistry: The preparation and properties of a specific polysaccharide from B. dysenteriae (Shiga). Biochem J. 1936 May;30(5):909–925. doi: 10.1042/bj0300909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUDIN J. B. Specific precipitation in gels and its application to immunochemical analysis. Methods Med Res. 1952;5:335–378. [PubMed] [Google Scholar]
- PILLEMER L., SCHOENBERG M. D., BLUM L., WURZ L. Properdin system and immunity. II. Interaction of the properdin system with polysaccharides. Science. 1955 Sep 23;122(3169):545–549. doi: 10.1126/science.122.3169.545. [DOI] [PubMed] [Google Scholar]
- TAL C., GOEBEL W. F. On the nature of the toxic component of the somatic antigen of Shigella paradysenteriae type Z (Flexner). J Exp Med. 1950 Jul 1;92(1):25–34. doi: 10.1084/jem.92.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]