Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1961 Sep 30;114(4):509–520. doi: 10.1084/jem.114.4.509

THE ROLE OF HUMORAL ANTIBODIES IN REJECTION OF SKIN HOMOGRAFTS IN RABBITS

I. PASSIVE TRANSFER OF ISOIMMUNE SERUM TO CONDITIONED HOSTS

Roberto R Kretschmer 1, Ruy Peréz-Tamayo 1
PMCID: PMC2180364  PMID: 19867199

Abstract

Gross and microscopic observations of skin homograft rejection carried out in cortisone-conditioned and non-conditioned rabbits seem to indicate that humoral antibodies play an important role in the phenomenon. Thus, local administration of isoimmune serum to animals bearing skin homografts resulted in a significantly earlier rejection of that particular test graft without modifying the course of a neighboring control-skin graft. This result appears to support the idea that homograft rejection is not only due to cellular antibodies but to a combination of both humoral and cellular immune responses, which should not be regarded as completely unrelated.

Full Text

The Full Text of this article is available as a PDF (935.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALGIRE G. H. Growth inhibition of homografts of a plasma-cell neoplasm in cell-impenetrable diffusion chambers placed in hyperimmunized mice. J Natl Cancer Inst. 1959 Sep;23:435–439. [PubMed] [Google Scholar]
  2. ALGIRE G. H., WEAVER J. M., PREHN R. T. Growth of cells in vivo in diffusion chambers. I. Survival of homografts in immunized mice. J Natl Cancer Inst. 1954 Dec;15(3):493–507. [PubMed] [Google Scholar]
  3. ALLGOWER M., BLOCKER T. G., Jr, ENGLEY B. W. D. Some immunological aspects of auto- and homografts in rabbits, tested by in vivo and in vitro techniques. Plast Reconstr Surg (1946) 1952 Jan;9(1):1–21. [PubMed] [Google Scholar]
  4. AMOS D. B., GORER P. A., MIKULSKA B. M., BILLINGHAM R. E., SPARROW E. M. An antibody response to skin homografts in mice. Br J Exp Pathol. 1954 Apr;35(2):203–208. [PMC free article] [PubMed] [Google Scholar]
  5. AMOS D. B. The persistence of mouse iso-antibodies in vivo. Br J Cancer. 1955 Mar;9(1):216–221. doi: 10.1038/bjc.1955.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. AMOS D. B., WAKEFIELD J. D. Growth of mouse ascites tumor cells in diffusion chambers. II. Lysis and growth inhibition by diffusible iso-antibody. J Natl Cancer Inst. 1959 Jun;22(6):1077–1092. [PubMed] [Google Scholar]
  7. BERRIAN J. H., BRENT L. Cell-bound antibodies in transplantation immunity. Ann N Y Acad Sci. 1958 Oct 7;73(3):654–662. doi: 10.1111/j.1749-6632.1959.tb40841.x. [DOI] [PubMed] [Google Scholar]
  8. BILLINGHAM R. E., BRENT L. Further attempts to transfer transplantation immunity by means of serum. Br J Exp Pathol. 1956 Dec;37(6):566–569. [PMC free article] [PubMed] [Google Scholar]
  9. BILLINGHAM R. E., BRENT L., MEDAWAR P. B. Actively acquired tolerance of foreign cells. Nature. 1953 Oct 3;172(4379):603–606. doi: 10.1038/172603a0. [DOI] [PubMed] [Google Scholar]
  10. BILLINGHAM R. E., BRENT L., MEDAWAR P. B. The antigenic stimulus in transplantation immunity. Nature. 1956 Sep 8;178(4532):514–519. doi: 10.1038/178514a0. [DOI] [PubMed] [Google Scholar]
  11. BILLINGHAM R. E., KROHN P. L., MEDAWAR P. B. Effect of locally applied cortisone acetate on survival of skin homografts in rabbits. Br Med J. 1951 Nov 3;2(4739):1049–1053. doi: 10.1136/bmj.2.4739.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. BRENT L. Tissue transplantation immunity. Prog Allergy. 1958;5:271–348. [PubMed] [Google Scholar]
  13. DAMMIN G. J., MURRAY J. E. Criteria for acceptance of skin grafts. Transplant Bull. 1959 Oct;6:429–432. doi: 10.1097/00006534-195910000-00041. [DOI] [PubMed] [Google Scholar]
  14. GARVER R. M., COLE L. J. Passive transfer of bone marrow homotransplantation immunity with specific antisera. J Immunol. 1961 Mar;86:307–311. [PubMed] [Google Scholar]
  15. GOOD R. A., VARCO R. L., AUST J. B., ZAK S. J. Transplantation studies in patients with agammaglobulinemia. Ann N Y Acad Sci. 1957 Mar 22;64(5):882-924; discussion, 924-8. doi: 10.1111/j.1749-6632.1957.tb52482.x. [DOI] [PubMed] [Google Scholar]
  16. GOOD R. A., VARCO R. L. Successful homograft of skin in a child with agammaglobulinemia; studies on agammaglobulinemia. J Am Med Assoc. 1955 Feb 26;157(9):713–716. doi: 10.1001/jama.1955.02950260019004c. [DOI] [PubMed] [Google Scholar]
  17. GORER P. A. Some recent work on tumor immunity. Adv Cancer Res. 1956;4:149–186. doi: 10.1016/s0065-230x(08)60724-1. [DOI] [PubMed] [Google Scholar]
  18. HARRIS S., HARRIS T. N., FARBER M. B. Studies on the transfer of lymph node cells. XII. The effect of anti-rabbit-leucocyte serum on the transfer of antigen-incubated lymph node cells. J Exp Med. 1958 Oct 1;108(4):411–429. doi: 10.1084/jem.108.4.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. HOLUB M. Antibody production by lymphocytes after in vitro contact with bacterial antigen and transfer to new-born rabbits. Nature. 1958 Jan 11;181(4602):122–122. doi: 10.1038/181122a0. [DOI] [PubMed] [Google Scholar]
  20. JENSEN E., STETSON C. A., Jr Humoral aspects of the immune response to homografts. II. Relationship between the hemagglutinating and cytotoxic activities of certain isoimmune sera. J Exp Med. 1961 Apr 1;113:785–794. doi: 10.1084/jem.113.4.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LAWRENCE H. S. Homograft sensitivity. An expression of the immunologic origins and consequences of individuality. Physiol Rev. 1959 Oct;39:811–859. doi: 10.1152/physrev.1959.39.4.811. [DOI] [PubMed] [Google Scholar]
  22. LAWRENCE H. S., RAPAPORT F. T., CONVERSE J. M., TILLETT W. S. Transfer of delayed hypersensitivity to skin homografts with leukocyte extracts in man. J Clin Invest. 1960 Jan;39:185–198. doi: 10.1172/JCI104018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. MITCHISON N. A. Studies on the immunological response to foreign tumor transplants in the mouse. I. The role of lymph node cells in conferring immunity by adoptive transfer. J Exp Med. 1955 Aug 1;102(2):157–177. doi: 10.1084/jem.102.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. ROBERTS J. C., Jr, DIXON F. J., WEIGLE W. O. Antibody-producing lymph node cells and peritoneal exudate cells; morphologic studies of transfers to immunologically inert rabbits. AMA Arch Pathol. 1957 Sep;64(3):324–332. [PubMed] [Google Scholar]
  25. SCOTHORNE R. J. Studies on the response of the regional lymph node to skin homografts. Ann N Y Acad Sci. 1957 Mar 22;64(5):1028-38; discussion, 1038-9. doi: 10.1111/j.1749-6632.1957.tb52494.x. [DOI] [PubMed] [Google Scholar]
  26. SIMONSEN M., BUEMANN J., GAMMELTOFT A., JENSEN F., JORGENSEN K. Biological incompatibility in kidney transplantation in dogs. I. Experimental and morphological investigations. Acta Pathol Microbiol Scand. 1953;32(1):1–35. doi: 10.1111/j.1699-0463.1953.tb00229.x. [DOI] [PubMed] [Google Scholar]
  27. STETSON C. A., Jr, DEMOPOULOS R. Reactions of skin homografts with specific immune sera. Ann N Y Acad Sci. 1958 Oct 7;73(3):687–692. doi: 10.1111/j.1749-6632.1959.tb40845.x. [DOI] [PubMed] [Google Scholar]
  28. TERASAKI P. I., CANNON J. A., LONGMIRE W. P., Jr, CHAMBERLAIN C. C. Antibody response to homografts: V. Cytotoxic effects upon lymphocytes as measured by time-lapse cinematography. Ann N Y Acad Sci. 1960 May 31;87:258–265. doi: 10.1111/j.1749-6632.1960.tb23199.x. [DOI] [PubMed] [Google Scholar]
  29. WEAVER J. M., ALGIRE G. H., PREHN R. T. The growth of cells in vivo in diffusion chambers. II. The role of cells in the destruction of homografts in mice. J Natl Cancer Inst. 1955 Jun;15(6):1737–1767. [PubMed] [Google Scholar]
  30. WOODRUFF M. F. Cellular and humoral factors in the immunity to skin homografts: experiments with a porous membrane. Ann N Y Acad Sci. 1957 Mar 22;64(5):1014-26; discussion, 1026-7. doi: 10.1111/j.1749-6632.1957.tb52493.x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES