Abstract
Total serum hemolytic complement activity is consistently reduced in animals undergoing accelerated "serum sickness". The time of maximum reduction in complement titer invariably coincides with the time of complete antigen disappearance. This temporal relationship supports the belief that the reduction in complement titer is brought about by in vivo antigen-antibody combination. The intravenous administration of a single daily large dose of BGG-precipitated complexes produces a marked but temporary reduction in total complement hemolytic activity. On the other hand, multiple injections of soluble BGG immune complexes prepared in antigen excess failed to even temporarily alter complement titers. Animals undergoing the development of accelerated "serum sickness" treated by either type immune complex showed a significantly reduced incidence of arterial lesions. Apparently, this inhibiting effect on the development of arterial lesions was independent of variations in total complement hemolytic activity.
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Selected References
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- BECKER E. L. Concerning the mechanism of complement action. I. Inhibition of complement activity by diisopropyl fluophosphate. J Immunol. 1956 Dec;77(6):462–468. [PubMed] [Google Scholar]
- DIXON F. J., FELDMAN J. D., VAZQUEZ J. J. Experimental glomerulonephritis. The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis. J Exp Med. 1961 May 1;113:899–920. doi: 10.1084/jem.113.5.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERMUTH F. G., Jr A comparative histologic and immunologic study in rabbits of induced hypersensitivity of the serum sickness type. J Exp Med. 1953 Feb 1;97(2):257–282. doi: 10.1084/jem.97.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERMUTH F. G., Jr, FLANAGAN C., MONTENEGRO M. R. The relationships between the chemical nature of the antigen, antigen dosage, rate of antibody synthesis and the occurrence of arteritis and glomerulonephritis in experimental hypersensitivity. Bull Johns Hopkins Hosp. 1957 Sep;101(3):149–169. [PubMed] [Google Scholar]
- GERMUTH F. G., Jr, MCKINNON G. E. Studies on the biological properties of antigen-antibody complexes. I. Anaphylatic shock induced by soluble antigen-antibody complexes in unsensitized normal guinea pigs. Bull Johns Hopkins Hosp. 1957 Jul;101(1):13–44. [PubMed] [Google Scholar]
- LEPOW I. H., RATNOFF O. D., PILLEMER L. Elution of an esterase from antigen-antibody aggregates treated with human complement. Proc Soc Exp Biol Med. 1956 May;92(1):111–114. doi: 10.3181/00379727-92-22401. [DOI] [PubMed] [Google Scholar]
- LEVINE L. Inhibition of immune hemolysis by diisopropyl fluorophosphate. Biochim Biophys Acta. 1955 Oct;18(2):283–284. doi: 10.1016/0006-3002(55)90069-x. [DOI] [PubMed] [Google Scholar]
- MOLL F. C., HAWN C. V. Z. Experimental hypersensitivity; relationship of dosage to serological and pathological responses following injection of heterologous protein. Proc Soc Exp Biol Med. 1952 May;80(1):77–81. doi: 10.3181/00379727-80-19529. [DOI] [PubMed] [Google Scholar]
- OSLER A. G., HAWRISIAK M. M., OVARY Z., SIQUEIRA M., BIER O. G. Studies on the mechanism of hypersensitivity phenomena. II. The participation of complement in passive cutaneous anaphylaxis of the albino rat. J Exp Med. 1957 Dec 1;106(6):811–834. doi: 10.1084/jem.106.6.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OSLER A. G., RANDALL H. G., HILL B. M., OVARY Z. Studies on the mechanism of hypersensitivity phenomena. III. The participation of complement in the formation of anaphylatoxin. J Exp Med. 1959 Aug 1;110(2):311–339. doi: 10.1084/jem.110.2.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RICE C. E. An investigation of some of the factors determining the decrease in complement activity in anaphylactic shock. J Immunol. 1955 Aug;75(2):85–95. [PubMed] [Google Scholar]
- SCHWAB L., MOLL F. C., HALL T., BREAN H., KIRK M., HAWN C. van Z., HANEWAY C. A. Experimental hypersensitivity in the rabbit; effect of inhibition of antibody formation by X-radiation and nitrogen mustards on the histologic and serologic sequences, and on the behavior of serum complement, following single large injections of foreign Proteins. J Exp Med. 1950 May 1;91(5):505–526. doi: 10.1084/jem.91.5.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAVITSKY A. B., STAVITSKY R., ECKER E. E. Loss of hemolytic-complement activity and of granulocytes following reinjection of an antigen into the rabbit. J Immunol. 1949 Dec;63(4):389–407. [PubMed] [Google Scholar]
- WEIGLE W. O., COCHRANE C. G., DIXON F. J. Anaphylactogenic properties of soluble antigen-antibody complexes in the guinea pig and rabbit. J Immunol. 1960 Nov;85:469–477. [PubMed] [Google Scholar]