Abstract
A temperature-sensitive Bacillus subtilis initiation mutant was used to achieve one cycle of synchronized deoxyribonucleic acid (DNA) replication. Markers near the origin of replication and the terminus were assayed for association with the cell membrane at intervals during the DNA replication cycle. DNA near the origin and terminus was found to be enriched in the membrane fraction throughout the DNA replication cycle. The magnitude of membrane enrichment or origin and terminus markers varied coincidentally, possibly as a consequence of incubating the cells at 45 degrees C.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bott K. F., Wilson G. A. Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967 Sep;94(3):562–570. doi: 10.1128/jb.94.3.562-570.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drlica K., Burgi E., Worcel A. Association of the folded chromosome with the cell envelope of Escherichia coli: nature of the membrane-associated DNA. J Bacteriol. 1978 Jun;134(3):1108–1116. doi: 10.1128/jb.134.3.1108-1116.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fielding P., Fox C. F. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli. Biochem Biophys Res Commun. 1970 Oct 9;41(1):157–162. doi: 10.1016/0006-291x(70)90482-1. [DOI] [PubMed] [Google Scholar]
- Jones N. C., Donachie W. D. Protein synthesis and the release of the replicated chromosome from the cell membrane. Nature. 1974 Sep 20;251(5472):252–254. doi: 10.1038/251252a0. [DOI] [PubMed] [Google Scholar]
- Korch C., Ovrebo S., Kleppe K. Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions. J Bacteriol. 1976 Aug;127(2):904–916. doi: 10.1128/jb.127.2.904-916.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
- O'Sullivan M. A., Sueoka N. Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis. J Mol Biol. 1972 Aug 21;69(2):237–248. doi: 10.1016/0022-2836(72)90228-8. [DOI] [PubMed] [Google Scholar]
- OISHI M., YOSHIKAWA H., SUEOKA N. SYNCHRONOUS AND DICHOTOMOUS REPLICATIONS OF THE BACILLUS SUBTILIS CHROMOSOME DURING SPORE GERMINATION. Nature. 1964 Dec 12;204:1069–1073. doi: 10.1038/2041069a0. [DOI] [PubMed] [Google Scholar]
- Parker D. L., Glaser D. A. Chromosomal sites of DNA-membrane attachment in Escherichia coli. J Mol Biol. 1974 Aug 5;87(2):153–168. doi: 10.1016/0022-2836(74)90140-5. [DOI] [PubMed] [Google Scholar]
- Ryder O. A., Smith D. W. Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition. J Bacteriol. 1974 Dec;120(3):1356–1363. doi: 10.1128/jb.120.3.1356-1363.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryder O. A., Smith D. W. Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication. Cell. 1975 Apr;4(4):337–345. doi: 10.1016/0092-8674(75)90154-3. [DOI] [PubMed] [Google Scholar]
- Snyder R. W., Young F. E. Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis. Biochem Biophys Res Commun. 1969 May 8;35(3):354–362. doi: 10.1016/0006-291x(69)90506-3. [DOI] [PubMed] [Google Scholar]
- Sueoka N., Hammers J. M. Isolation of DNA-membrane complex in Bacillus subtilis. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4787–4791. doi: 10.1073/pnas.71.12.4787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
- White K., Sueoka N. Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication. Genetics. 1973 Feb;73(2):185–214. doi: 10.1093/genetics/73.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Worcel A., Burgi E. Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol. 1974 Jan 5;82(1):91–105. doi: 10.1016/0022-2836(74)90576-2. [DOI] [PubMed] [Google Scholar]
- YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamaguchi K., Yoshikawa H. Association of the replication terminus of the Bacillus subtilis chromosome to the cell membrane. J Bacteriol. 1975 Nov;124(2):1030–1033. doi: 10.1128/jb.124.2.1030-1033.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamaguchi K., Yoshikawa H. Topography of chromosome membrane junction in Bacillus subtilis. Nat New Biol. 1973 Aug 15;244(137):204–206. doi: 10.1038/newbio244204a0. [DOI] [PubMed] [Google Scholar]