Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1961 Nov 30;114(6):857–874. doi: 10.1084/jem.114.6.857

EFFECTS OF BACTERIAL ENDOTOXIN ON RABBIT PLATELETS

I. PLATELET AGGREGATION AND RELEASE OF PLATELET FACTORS IN VITRO

Roger M Des Prez 1, Herbert I Horowitz 1, Edward W Hook 1
PMCID: PMC2180403  PMID: 13885652

Abstract

Incubation of platelet-rich rabbit plasma with E. coli endotoxin at 37°C results in platelet aggregation and transfer of platelet 5-hydroxytryptamine to plasma. Release of 5HT is influenced by dose of endotoxin, type of anticoagulant, and temperature of incubation. A heat-labile plasma factor is necessary for the platelet-endotoxin interaction. Additional studies have shown that incubation of endotoxin with platelet-rich rabbit plasma also results in release of platelet phospholipid and bactericidins active against B. subtilis.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG D., JEPSON J. B., KEELE C. A., STEWART J. W. Pain-producing substance in human inflammatory exudates and plasma. J Physiol. 1957 Feb 15;135(2):350–370. doi: 10.1113/jphysiol.1957.sp005715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARBARO J. F. The release of histamine from rabbit platelets by means of antigen-antibody precipitates. I. The participation of the immune complex in histamine release. J Immunol. 1961 Apr;86:369–376. [PubMed] [Google Scholar]
  3. BARBARO J. F. The release of histamine from rabbit platelets by means of antigen-antibody precipitates. II. The role of plasma in the release of histamine. J Immunol. 1961 Apr;86:377–381. [PubMed] [Google Scholar]
  4. BELL W. N., ALTON H. G. A brain extract as a substitute for platelet suspensions in the thromboplastin generation test. Nature. 1954 Nov 6;174(4436):880–881. doi: 10.1038/174880a0. [DOI] [PubMed] [Google Scholar]
  5. BERGSAGEL D. E., HOUGIE C. Intermediate stages in the formation of blood thromboplastin. Br J Haematol. 1956 Apr;2(2):113–129. doi: 10.1111/j.1365-2141.1956.tb06820.x. [DOI] [PubMed] [Google Scholar]
  6. BORST H. G., BERGLUND E., MCGREGOR M. The effects of pharmacologic agents on the pulmonary circulation in the dog; studies on epinephrine, nor-epinephrine, 5-hydroxytryptamine, acetylcholine, histamine and aminophylline. J Clin Invest. 1957 May;36(5):669–675. doi: 10.1172/JCI103467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAVIS R. B., MEEKER W. R., McQUARRIE D. G. Immediate effects of intravenous endotoxin on serotonin concentrations and blood platelets. Circ Res. 1960 Jan;8:234–239. doi: 10.1161/01.res.8.1.234. [DOI] [PubMed] [Google Scholar]
  8. ERSPAMER V. Pharmacology of indole-alkylamines. Pharmacol Rev. 1954 Dec;6(4):425–487. [PubMed] [Google Scholar]
  9. GILBERT R. P. Mechanisms of the hemodynamic effects of endotoxin. Physiol Rev. 1960 Apr;40:245–279. doi: 10.1152/physrev.1960.40.2.245. [DOI] [PubMed] [Google Scholar]
  10. HINSHAW L. B., KUIDA H., GILBERT R. P., VISSCHER M. B. Influence of perfusate characteristics on pulmonary vascular response to endotoxin. Am J Physiol. 1957 Nov;191(2):293–295. doi: 10.1152/ajplegacy.1957.191.2.293. [DOI] [PubMed] [Google Scholar]
  11. HIRSCH J. G. Comparative bactericidal activities of blood serum and plasma serum. J Exp Med. 1960 Jul 1;112:15–22. doi: 10.1084/jem.112.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HOROWITZ H. I., SPAET T. H. Generation of coagulation product I and its interaction with platelets and phospholipids. J Appl Physiol. 1961 Jan;16:112–114. doi: 10.1152/jappl.1961.16.1.112. [DOI] [PubMed] [Google Scholar]
  13. HUMPHREY J. H., JAQUES R. The release of histamine and 5-hydroxytryptamine (serotonin) from platelets by antigen-antibody reactions (in vitro). J Physiol. 1955 Apr 28;128(1):9–27. doi: 10.1113/jphysiol.1955.sp005289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. JAGO R., JACOX R. F. Cellular source and charcter of a heatstable bactericidal property associated with rabbit and rat platelets. J Exp Med. 1961 Apr 1;113:701–711. doi: 10.1084/jem.113.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KABINS S. A., MOLINA C., KATZ L. N. Pulmonary vascular effects of serotonin (5-OH-tryptamine) in dogs: its role in causing pulmonary edema. Am J Physiol. 1959 Nov;197:955–958. doi: 10.1152/ajplegacy.1959.197.5.955. [DOI] [PubMed] [Google Scholar]
  16. LEWIS G. P. Formation of plasma kinins by plasmin. J Physiol. 1958 Feb 17;140(2):285–300. doi: 10.1113/jphysiol.1958.sp005934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MARGOLIS J. Initiation of blood coagulation by glass and related surfaces. J Physiol. 1957 Jun 18;137(1):95–109. doi: 10.1113/jphysiol.1957.sp005799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McKAY D. G., SHAPIRO S. S. Alterations in the blood coagulation system induced by bacterial endotoxin. I. In vivo (generalized Shwartzman reaction). J Exp Med. 1958 Mar 1;107(3):353–367. doi: 10.1084/jem.107.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McKAY D. G., SHAPIRO S. S., SHANBERGE J. N. Alterations in the blood coagulation system induced by bacterial endotoxins. II. In vitro. J Exp Med. 1958 Mar 1;107(3):369–376. doi: 10.1084/jem.107.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ROBBINS J., STETSON C. A., Jr An effect of antigen-antibody interaction on blood coagulation. J Exp Med. 1959 Jan 1;109(1):1–8. doi: 10.1084/jem.109.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STETSON C. A., Jr Studies on the mechanism of the Shwartzman phenomenon; certain factors involved in the production of the local hemorrhagic necrosis. J Exp Med. 1951 May;93(5):489–504. doi: 10.1084/jem.93.5.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. THOMAS L., ZWEIFACH B. W., BENACERRAF B. Mechanisms in the production of tissue damage and shock by endotoxins. Trans Assoc Am Physicians. 1957;70:54–63. [PubMed] [Google Scholar]
  23. WAALKES T. P., WEISSBACH H., BOZICEVICH J., UDENFRIEND S. Serotonin and histamine release during anaphylaxis in the rabbit. J Clin Invest. 1957 Jul;36(7):1115–1120. doi: 10.1172/JCI103507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WOOLLEY D. W., SHAW E. Differentiation between receptors for serotonin and tryptamine by means of the exquisite specificity of antimetabolites. J Pharmacol Exp Ther. 1957 Sep;121(1):13–17. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES