Abstract
The uptake of intravenously injected coconut oil emulsion by the liver and spleen was investigated by gas-liquid chromatography in normal, triton-injected, and hypercholesterolemic rabbits. The lauric and myristic acids from this emulsion, almost absent in the tissues of normal animals, were used as marked acids. In normal rabbits only the liver took up a quantitatively important fraction of the injected fat. The uptake by the spleen appears to be due to a different mechanism than that of the liver. In rabbits given triton the liver was incapable of taking up large quantities of fat, while the uptake in the spleen was undisturbed. 12 hours after injection the plasma, liver, and spleen of normal animals contained only 14 per cent of the dose. With animals given triton 71 per cent of the injected dose was recovered, most of it in the plasma. Hypercholesterolemic rabbits cleared an injected fat emulsion and took fat into the liver at the same rate as the controls. When triton is injected 6 hours after the administration of the emulsion, the fat taken up in the liver reappears in the plasma.
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Selected References
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- BROWN R. K., BOYLE E., ANFINSEN C. B. The enzymatic transformation of lipoproteins. J Biol Chem. 1953 Sep;204(1):423–434. [PubMed] [Google Scholar]
- BYERS S. O., FRIEDMAN M. Site of origin of plasma triglyceride. Am J Physiol. 1960 Mar;198:629–631. doi: 10.1152/ajplegacy.1960.198.3.629. [DOI] [PubMed] [Google Scholar]
- EDGREN B. The removal of artificial fat emulsions from the blood stream of dogs. Acta Physiol Scand. 1960 Apr 25;48:390–401. doi: 10.1111/j.1748-1716.1960.tb01872.x. [DOI] [PubMed] [Google Scholar]
- FRIEDMAN M., BYERS S. O. The mechanism responsible for the hypercholesteremia induced by triton WR-1339. J Exp Med. 1953 Jan;97(1):117–130. doi: 10.1084/jem.97.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HIRSCH J., AHRENS E. H., Jr The separation of complex lipide mixtures by the use of silicic acid chromatography. J Biol Chem. 1958 Aug;233(2):311–20. [PubMed] [Google Scholar]
- HIRSCH R. L., KELLNER A. The pathogenesis of hyperlipemia induced by means of surface-active agents. II. Failure of exchange of cholesterol between the plasma and the liver in rabbits given triton WR 1339. J Exp Med. 1956 Jul 1;104(1):15–24. doi: 10.1084/jem.104.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MURRAY R. G., FREEMAN S. The morphologic distribution of intravenously injected fatty chyle and artificial fat emulsion in rats and dogs. J Lab Clin Med. 1951 Jul;38(1):56–69. [PubMed] [Google Scholar]
- SCANU A., ORIENTE P. Triton hyperlipemia in dogs. I. In vitro effects of the detergent on serum lipoproteins and chylomcrons. J Exp Med. 1961 Apr 1;113:735–757. doi: 10.1084/jem.113.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHOTZ M. C., SCANU A., PAGE I. H. Effect of triton on lipoprotein lipase of rat plasma. Am J Physiol. 1957 Feb;188(2):399–402. doi: 10.1152/ajplegacy.1957.188.2.399. [DOI] [PubMed] [Google Scholar]
- VAN DEN BOSCH J., EVRARD E., BILLIAU A., DE SOMER P., JOOSSENS J. V. The influence of a new p-tert-octylphenol derivative on the clearing factor and on the plasma lipids of rabbits. J Atheroscler Res. 1961 Mar-Apr;1:148–162. doi: 10.1016/s0368-1319(61)80044-6. [DOI] [PubMed] [Google Scholar]
- WADDELL W. R., GEYER R. P., CLARKE E., STARE F. J. Function of the reticuloendothelial system in removal of emulsified fat from blood. Am J Physiol. 1954 Apr;177(1):90–94. doi: 10.1152/ajplegacy.1954.177.1.90. [DOI] [PubMed] [Google Scholar]
- WADDELL W. R., GEYER R. P., CLARKE E., STARE F. J. Role of various organs in the removal of emulsified fat from the blood stream. Am J Physiol. 1953 Nov;175(2):299–302. doi: 10.1152/ajplegacy.1953.175.2.299. [DOI] [PubMed] [Google Scholar]
- ZLATKIS A., ZAK B., BOYLE A. J. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953 Mar;41(3):486–492. [PubMed] [Google Scholar]