Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Sep;139(3):961–976. doi: 10.1128/jb.139.3.961-976.1979

Specificity in formation of type II F' plasmids.

R G Hadley, R C Deonier
PMCID: PMC218044  PMID: 383701

Abstract

Eight new F' plasmids derived from Hfr strains in which F is integrated at the chromosomal element alpha 3 beta 3 have been isolated and subjected to restriction enzyme, hybridization, and electron microscope heteroduplex analysis. Plasmids carrying extensive amounts of bacterial deoxyribonucleic acid were produced even though they were obtained by selection for transfer of lac, which is closely linked to F in the parental Hfr strains. Seven plasmids were type II Flac+ proC+ purE+ plasmids, and one was a type I Flac+ proC+ plasmid. Five of the Flac+ proC+ purE+ plasmids contain approximately 284 kilobases of bacterial deoxyribonucleic acid, which is identical for all five within the resolution of the restriction enzyme analysis. Theses results indicate that type II F' plasmids are the predominant tra+ F' type from this region of the Escherichia coli K-12 chromosome and that the recombination events leading to formation of these plasmids exhibit site specificity.

Full text

PDF

Images in this article

966Image
on p.966
966Image
on p.966
967Image
on p.967
972Image
on p.972
972Image
on p.972

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Skurray R. A., Thompson R., Helmuth R., Hall S., Beutin L., Clark A. J. Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol. 1978 Mar;133(3):1383–1392. doi: 10.1128/jb.133.3.1383-1392.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bazaral M., Helinski D. R. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185–194. doi: 10.1016/0022-2836(68)90374-4. [DOI] [PubMed] [Google Scholar]
  3. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  4. Broda P., Meacock P. Isolation and characterisation of Hfr strains from a recombination-deficient strain of Escherichia coli. Mol Gen Genet. 1971;113(2):166–173. doi: 10.1007/BF00333190. [DOI] [PubMed] [Google Scholar]
  5. Childs G. J., Ohtsubo H., Ohtsubo E., Sonnenberg F., Freundlich M. Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes. J Mol Biol. 1977 Nov 25;117(1):175–193. doi: 10.1016/0022-2836(77)90030-4. [DOI] [PubMed] [Google Scholar]
  6. Clarke L., Carbon J. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976 Sep;9(1):91–99. doi: 10.1016/0092-8674(76)90055-6. [DOI] [PubMed] [Google Scholar]
  7. Curtiss R., 3rd, Stallions D. R. Probability of F integration and frequency of stable Hfr donors in F+ populations of Escherichia coli K-12. Genetics. 1969 Sep;63(1):27–38. doi: 10.1093/genetics/63.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  9. Deonier R. C., Davidson N. The sequence organization of the integrated F plasmid in two Hfr strains of Escherichia coli. J Mol Biol. 1976 Nov 5;107(3):207–222. doi: 10.1016/s0022-2836(76)80002-2. [DOI] [PubMed] [Google Scholar]
  10. Deonier R. C., Hadley R. G., Hu M. Enumeration and identification of IS3 elements in Escherichia coli strains. J Bacteriol. 1979 Mar;137(3):1421–1424. doi: 10.1128/jb.137.3.1421-1424.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Deonier R. C., Mirels L. Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3965–3969. doi: 10.1073/pnas.74.9.3965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Deonier R. C., Oh G. R., Hu M. Further mapping of IS2 and IS3 in the lac-purE region of the Escherichia coli K-12 genome: structure of the F-prime ORF203. J Bacteriol. 1977 Feb;129(2):1129–1140. doi: 10.1128/jb.129.2.1129-1140.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Dewitt S K, Adelberg E A. The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics. 1962 May;47(5):577–585. doi: 10.1093/genetics/47.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Engel J. D., Dodgson J. B. Analysis of the adult and embryonic chicken globin genes in chromosomal DNA. J Biol Chem. 1978 Nov 25;253(22):8239–8246. [PubMed] [Google Scholar]
  15. Hoppe I., Roth J. Specialized transducing phages derived from salmonella phage P22. Genetics. 1974 Apr;76(4):633–654. doi: 10.1093/genetics/76.4.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hu S., Ohtsubo E., Davidson N. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. J Bacteriol. 1975 May;122(2):749–763. doi: 10.1128/jb.122.2.749-763.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hu S., Otsubo E., Davidson N., Saedler H. Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J Bacteriol. 1975 May;122(2):764–775. doi: 10.1128/jb.122.2.764-775.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  20. Landy A., Ross W. Viral integration and excision: structure of the lambda att sites. Science. 1977 Sep 16;197(4309):1147–1160. doi: 10.1126/science.331474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  22. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Low K. B., Porter D. D. Modes of gene transfer and recombination in bacteria. Annu Rev Genet. 1978;12:249–287. doi: 10.1146/annurev.ge.12.120178.001341. [DOI] [PubMed] [Google Scholar]
  24. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  25. Morrow J. F., Cohen S. N., Chang A. C., Boyer H. W., Goodman H. M., Helling R. B. Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1974 May;71(5):1743–1747. doi: 10.1073/pnas.71.5.1743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ohtsubo E., Hsu M. T. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: isolation of a new F-prime factor, F80, and its implication for the mechanism of F integration into the chromosome. J Bacteriol. 1978 Jun;134(3):795–800. doi: 10.1128/jb.134.3.795-800.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ohtsubo E., Hsu M. T. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J Bacteriol. 1978 Jun;134(3):778–794. doi: 10.1128/jb.134.3.778-794.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ptashne K., Cohen S. N. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol. 1975 May;122(2):776–781. doi: 10.1128/jb.122.2.776-781.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Reif H. J., Saedler H. IS1 is involved in deletion formation in the gal region of E. coli K12. Mol Gen Genet. 1975;137(1):17–28. doi: 10.1007/BF00332538. [DOI] [PubMed] [Google Scholar]
  30. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  31. Scaife J. Episomes. Annu Rev Microbiol. 1967;21:601–638. doi: 10.1146/annurev.mi.21.100167.003125. [DOI] [PubMed] [Google Scholar]
  32. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  33. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  34. Susskind M. M., Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev. 1978 Jun;42(2):385–413. doi: 10.1128/mr.42.2.385-413.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  36. Thompson R., Hughes S. G., Broda P. Plasmid identification using specific endonucleases. Mol Gen Genet. 1974;133(2):141–149. doi: 10.1007/BF00264835. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES