Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1966 Mar 31;123(4):599–614. doi: 10.1084/jem.123.4.599

CHARACTERISTICS OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODY ISOLATED FROM HYPERIMMUNE RABBITS

C Kirk Osterland 1, Edward J Miller 1, Walter W Karakawa 1, Richard M Krause 1
PMCID: PMC2180455  PMID: 4160396

Abstract

Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated γ-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of γ-globulin. The bulk of this γ-globulin, identified as γG-globulin, was homogeneous by zone electrophoresis and of specificity directed against the Group A-variant carbohydrate antigen. L chains isolated from specific antibody obtained from an immune precipitate were distributed as a single band on starch gel electrophoresis, whereas the normal γ-globulin traveled as a diffuse smear. These data suggest that the rabbit streptococcal Group A-variant antibodies possess a limited range of physicochemical properties and electrophoretic mobility compared to that generally observed for the normal complement of γ-globulin.

Full Text

The Full Text of this article is available as a PDF (778.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLLEN J. C., KUNKEL H. G., KABAT E. A. STUDIES ON HUMAN ANTIBODIES. II. DISTRIBUTION OF GENETIC FACTORS. J Exp Med. 1964 Mar 1;119:453–465. doi: 10.1084/jem.119.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ASKONAS B. A., FARTHING C. P., HUMPHREY J. H. The significance of multiple antibody components in serum of immunized rabbits. Immunology. 1960 Oct;3:336–351. [PMC free article] [PubMed] [Google Scholar]
  3. EDELMAN G. M., BENACERRAF B., OVARY Z. STRUCTURE AND SPECIFICITY OF GUINEA PIG 7S ANTIBODIES. J Exp Med. 1963 Aug 1;118:229–244. doi: 10.1084/jem.118.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EDELMAN G. M., POULIK M. D. Studies on structural units of the gamma-globulins. J Exp Med. 1961 May 1;113:861–884. doi: 10.1084/jem.113.5.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  6. HARBOE M., OSTERLAND C. K., MANNIK M., KUNKEL H. G. Genetic characters of human gamma-globulins in myeloma proteins. J Exp Med. 1962 Nov 1;116:719–738. doi: 10.1084/jem.116.5.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KARAKAWA W. W., OSTERLAND C. K., KRAUSE R. DETECTION OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODIES IN HUMAN SERA. J Exp Med. 1965 Aug 1;122:195–205. doi: 10.1084/jem.122.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KRAUSE R. M., MCCARTY M. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci. J Exp Med. 1961 Jul 1;114:127–140. doi: 10.1084/jem.114.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KRAUSE R. M. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. IV. ANTIGENIC AND BIOCHEMICAL COMPOSITION OF HEMOLYTIC STREPTOCOCCAL CELL WALLS. Bacteriol Rev. 1963 Dec;27:369–380. doi: 10.1128/br.27.4.369-380.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kunkel H. G., Mannik M., Williams R. C. Individual Antigenic Specificity of Isolated Antibodies. Science. 1963 Jun 14;140(3572):1218–1219. doi: 10.1126/science.140.3572.1218. [DOI] [PubMed] [Google Scholar]
  11. Lark C. A., Eisen H. N., Dray S. Distribution of allelic allotypes among gamma-G-immunoglobulins and purified anti-hapten antibodies from the same rabbit. J Immunol. 1965 Sep;95(3):404–411. [PubMed] [Google Scholar]
  12. MCCARTY M. Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates. J Exp Med. 1956 Nov 1;104(5):629–643. doi: 10.1084/jem.104.5.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. OUDIN J. Allotypy of rabbit serum proteins. I. Immuno-chemical analysis leading to the individualization of seven main allotypes. J Exp Med. 1960 Jul 1;112:107–124. doi: 10.1084/jem.112.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PORATH J., FLODIN P. Gel filtration: a method for desalting and group separation. Nature. 1959 Jun 13;183(4676):1657–1659. doi: 10.1038/1831657a0. [DOI] [PubMed] [Google Scholar]
  16. POULIK M. D. The use of urea-starch-gel electrophoresis in studies of reductive cleavage of an alpha 2-macroglobulin. Biochim Biophys Acta. 1960 Nov 4;44:390–393. doi: 10.1016/0006-3002(60)91590-0. [DOI] [PubMed] [Google Scholar]
  17. RIEDER R. F., OUDIN J. STUDIES ON THE RELATIONSHIP OF ALLOTYPIC SPECIFICITIES TO ANTIBODY SPECIFICITIES IN THE RABBIT. J Exp Med. 1963 Oct 1;118:627–633. doi: 10.1084/jem.118.4.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rotta J., Prendergast T. J., Karakawa W. W., Harmon C. K., Krause R. M. Enhanced resistance to streptococcal infection induced in mice by cell wall mucopeptide. J Exp Med. 1965 Nov 1;122(5):877–890. doi: 10.1084/jem.122.5.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  20. SMITHIES O. An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959 Mar;71(3):585–587. doi: 10.1042/bj0710585. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES