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. 1966 Mar 31;123(4):673–688. doi: 10.1084/jem.123.4.673

STUDIES ON THE EFFECT OF THE CARRIER MOLECULE ON ANTIHAPTEN ANTIBODY SYNTHESIS

I. EFFECT OF CARRIER ON THE NATURE OF THE ANTIBODY SYNTHESIZED

Gregory W Siskind 1, William E Paul 1, Baruj Benacerraf 1
PMCID: PMC2180458  PMID: 4160398

Abstract

1. Fluorescence quenching has been calibrated, by comparison with equilibrium dialysis, for measurement of antibody-hapten association constants with guinea pig antibody to the 2,4-dinitrophenyl (DNP) determinant. The maximum quenching with all sites occupied which gave best agreement with equilibrium dialysis was found to be 100%. 2. The carrier to which the DNP-hapten is coupled influences significantly the amount, class (γ1 or γ2), and affinity of the antibody of DNP specificity made by individual guinea pigs. 3. The affinities of γ1- and γ2-antibodies from individual guinea pigs are generally very similar. 4. Precipitin curves with γ1-antibodies show more marked inhibition of precipitation in antigen excess than do precipitin curves with the less charged γ2-antibodies, indicating the importance of nonspecific forces in the precipitation reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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