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. 1979 Sep;139(3):984–992. doi: 10.1128/jb.139.3.984-992.1979

High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol.

K Ochi, M J Hitchcock, E Katz
PMCID: PMC218046  PMID: 479112

Abstract

Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anne J., Eyssen H., De Somer P. Somatic hybridisation of Penicillium roquefortii with P. chrysogenum after protoplast fusion. Nature. 1976 Aug 19;262(5570):719–721. doi: 10.1038/262719a0. [DOI] [PubMed] [Google Scholar]
  2. Anné J., Peberdy J. F. Induced fusion of fungal protoplasts following treatment with polyethylene glycol. J Gen Microbiol. 1976 Feb;92(2):413–417. doi: 10.1099/00221287-92-2-413. [DOI] [PubMed] [Google Scholar]
  3. Baltz R. H. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J Gen Microbiol. 1978 Jul;107(1):93–102. doi: 10.1099/00221287-107-1-93. [DOI] [PubMed] [Google Scholar]
  4. Bibb M. J., Ward J. M., Hopwood D. A. Transformation of plasmid DNA into Streptomyces at high frequency. Nature. 1978 Jul 27;274(5669):398–400. doi: 10.1038/274398a0. [DOI] [PubMed] [Google Scholar]
  5. Delić V., Hopwood D. A., Friend E. J. Mutangenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutat Res. 1970 Feb;9(2):167–182. doi: 10.1016/0027-5107(70)90055-2. [DOI] [PubMed] [Google Scholar]
  6. Fodor K., Demiri E., Alföldi L. Polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of Bacillus megaterium. J Bacteriol. 1978 Jul;135(1):68–70. doi: 10.1128/jb.135.1.68-70.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GREGORY K. F., HUANG J. C. TYROSINASE INHERITANCE IN STREPTOMYCES SCABIES. I. GENETIC RECOMBINATION. J Bacteriol. 1964 Jun;87:1281–1286. doi: 10.1128/jb.87.6.1281-1286.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOPWOOD D. A., SERMONTI G., SPADA-SERMONTI I. Heterozygous clones in Streptomyces coelicolor. J Gen Microbiol. 1963 Feb;30:249–260. doi: 10.1099/00221287-30-2-249. [DOI] [PubMed] [Google Scholar]
  9. Hitchcock M. J., Katz E. Actinomycin biosynthesis by protoplasts derived from Streptomyces parvulus. Antimicrob Agents Chemother. 1978 Jan;13(1):104–114. doi: 10.1128/aac.13.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hopwood D. A. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967 Dec;31(4):373–403. doi: 10.1128/br.31.4.373-403.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hopwood D. A., Wright H. M. Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor. Mol Gen Genet. 1978 Jul 4;162(3):307–317. doi: 10.1007/BF00268856. [DOI] [PubMed] [Google Scholar]
  12. Hopwood D. A., Wright H. M., Bibb M. J., Cohen S. N. Genetic recombination through protoplast fusion in Streptomyces. Nature. 1977 Jul 14;268(5616):171–174. doi: 10.1038/268171a0. [DOI] [PubMed] [Google Scholar]
  13. KATZ E., GOSS W. A. Controlled biosynthesis of actinomycin with sarcosine. Biochem J. 1959 Nov;73:458–465. doi: 10.1042/bj0730458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SZYBALSKI W., BRYSON V. Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. J Bacteriol. 1952 Oct;64(4):489–499. doi: 10.1128/jb.64.4.489-499.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schaeffer P., Cami B., Hotchkiss R. D. Fusion of bacterial protoplasts. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151–2155. doi: 10.1073/pnas.73.6.2151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schaeffer P., Hotchkiss R. D. Fusion of bacterial protoplasts. Methods Cell Biol. 1978;20:149–158. doi: 10.1016/s0091-679x(08)62017-8. [DOI] [PubMed] [Google Scholar]
  17. Sipiczki M., Ferenczy L. Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type. Mol Gen Genet. 1977 Feb 28;151(1):77–81. doi: 10.1007/BF00446915. [DOI] [PubMed] [Google Scholar]
  18. van Solingen P., van der Plaat J. B. Fusion of yeast spheroplasts. J Bacteriol. 1977 May;130(2):946–947. doi: 10.1128/jb.130.2.946-947.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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