Abstract
High-rate antibody-forming cells and immunological memory cells can be selectively retained if filtered through a column coated with relevant antigen. This trapping can be blocked if the cells are incubated with an anti-immunoglobulin serum prior to column passage. A similar blocking is not observed when cells are treated with an anti-lymphocyte serum, thereby excluding the possibility that any antibodies combining with surface structures could cause this effect. By the use of antisera specific for heavy or light chain antigens, it was possible to locate such antigens in the antigen-binding receptor areas of immune cells. Criss-cross studies using antisera specific for gamma 1 or gamma 2a heavy chains showed that the membrane receptor has the same heavy chain as will be present in the eventual product of that cell, the humoral antibody.
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Selected References
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- Byrt P., Ada G. L. An in vitro reaction between labelled flagellin or haemocyanin and lymphocyte-like cells from normal animals. Immunology. 1969 Oct;17(4):503–516. [PMC free article] [PubMed] [Google Scholar]
- Coombs R. R., Feinstein A., Wilson A. B. Immunoglobulin determinants on the surface of human lymphocytes. Lancet. 1969 Nov 29;2(7631):1157–1160. doi: 10.1016/s0140-6736(69)92484-2. [DOI] [PubMed] [Google Scholar]
- Cross A. M., Mäkelä O. Selective inhibition of the secondary response of primed cells by incubation with hapten. Immunology. 1968 Sep;15(3):389–394. [PMC free article] [PubMed] [Google Scholar]
- MAKELA O., NOSSAL G. J. Bacterial adherence: a method for detecting antibody production by single cells. J Immunol. 1961 Oct;87:447–456. [PubMed] [Google Scholar]
- MUELLER A. P., WOLFE H. R., MEYER R. K., ASPINALL R. L. Further studies on the role of the bursa of Fabricius in antibody production. J Immunol. 1962 Mar;88:354–360. [PubMed] [Google Scholar]
- Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
- McConnell I., Munro A., Gurner B. W., Coombs R. R. Studies on actively allergized cells. I. The cyto-dynamics and morphology of rosete-forming lymph node cells in mice and inhibition of rosette-formation with antibody to mouse immunoglobulins. Int Arch Allergy Appl Immunol. 1969;35(3):209–227. [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mäkelä O., Mitchison N. A. The role of cell number and source in adoptive immunity. Immunology. 1965 Jun;8(6):539–548. [PMC free article] [PubMed] [Google Scholar]
- Naor D., Sulitzneau D. Binding of radioiodinated bovine serum albumin to mouse spleen cells. Nature. 1967 May 13;214(5089):687–688. doi: 10.1038/214687a0. [DOI] [PubMed] [Google Scholar]
- Pasanen V. J., Mäkelä O. Effect of the number of haptens coupled to each erythrocyte on haemolytic plaque formation. Immunology. 1969 Mar;16(3):399–407. [PMC free article] [PubMed] [Google Scholar]
- Raff M. C., Sternberg M., Taylor R. B. Immunoglobulin determinants on the surface of mouse lymphoid cells. Nature. 1970 Feb 7;225(5232):553–554. doi: 10.1038/225553a0. [DOI] [PubMed] [Google Scholar]
- Sell S., Gell P. G. Studies on rabbit lymphocytes in vitro. IV. Blast transformation of the lymphocytes from newborn rabbits induced by antiallotype serum to a paternal IgG allotype not present in the serum of the lymphocyte donors. J Exp Med. 1965 Nov 1;122(5):923–928. doi: 10.1084/jem.122.5.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shearer G. M., Cudkowicz G. Cellular differentiation of the immune system of mice. 3. Separate antigen-sensitive units for different types of anti-sheep immunocytes formed by marrow-thymus cell mixtures. J Exp Med. 1969 May 1;129(5):935–951. doi: 10.1084/jem.129.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
- Warner N. L., Byrt P., Ada G. L. Blocking of the lymphocyte antigen receptor site with anti-immunoglobulin sera in vitro. Nature. 1970 Jun 6;226(5249):942–943. doi: 10.1038/226942a0. [DOI] [PubMed] [Google Scholar]
- Wigzell H., Andersson B. Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells. J Exp Med. 1969 Jan 1;129(1):23–36. doi: 10.1084/jem.129.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wigzell H., Mäkelä O. Separation of normal and immune lymphoid cells by antigen-coated coated columns. Antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens. J Exp Med. 1970 Jul 1;132(1):110–126. doi: 10.1084/jem.132.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wigzell H., Möller G., Andersson B. Studies at the cellular level of the 19S immune response. Acta Pathol Microbiol Scand. 1966;66(4):530–540. doi: 10.1111/apm.1966.66.4.530. [DOI] [PubMed] [Google Scholar]
- Zaalberg O. B., van der Meul V. A., van Twisk M. J. Antibody production by isolated spleen cells: a study of the cluster and the plaque techniques. J Immunol. 1968 Feb;100(2):451–458. [PubMed] [Google Scholar]