Abstract
Heteroantisera were raised in rabbits against human thymocytes or Burkitt's lymphoma EBI cells. After suitable absorption, the sera were specific for thymocytes and T lymphoblasts, or B lymphoblasts and several lymphoblastoid cell lines. The specificity of the antisera was confirmed by preparing purified populations of T or B lymphocytes on immunoabsorbent columns, followed by mitogenic stimulation with phytohemagglutinin. The antisera did not react with normal resting lymphocytes.
Full Text
The Full Text of this article is available as a PDF (364.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Buell D. N., Fahey J. L. Limited periods of gene expression in immunoglobulin-synthesizing cells. Science. 1969 Jun 27;164(3887):1524–1525. doi: 10.1126/science.164.3887.1524. [DOI] [PubMed] [Google Scholar]
- Cikes M., Friberg S., Jr Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line. Proc Natl Acad Sci U S A. 1971 Mar;68(3):566–569. doi: 10.1073/pnas.68.3.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox T. O., Sheppard J. R., Burger M. M. Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis. Proc Natl Acad Sci U S A. 1971 Jan;68(1):244–247. doi: 10.1073/pnas.68.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halterman R. H., Leventhal B. G., Mann D. L. An acute-leukemia antigen: correlation with clinical status. N Engl J Med. 1972 Dec 21;287(25):1272–1274. doi: 10.1056/NEJM197212212872504. [DOI] [PubMed] [Google Scholar]
- Kuhns W. J., Bramson S. Variable behaviour of blood group H on HeLa cell populations synchronized with thymidine. Nature. 1968 Aug 31;219(5157):938–939. doi: 10.1038/219938a0. [DOI] [PubMed] [Google Scholar]
- Mayhew E. Cellular electrophoretic mobility and the mitotic cycle. J Gen Physiol. 1966 Mar;49(4):717–725. doi: 10.1085/jgp.49.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OLD L. J., BOYSE E. A. ANTIGENIC PROPERTIES OF EXPERIMENTAL LEUKEMIAS. I. SEROLOGICAL STUDIES IN VITRO WITH SPONTANEOUS AND RADIATION-INDUCED LEUKEMIAS. J Natl Cancer Inst. 1963 Oct;31:977–995. [PubMed] [Google Scholar]
- Pasternak C. A., Warmsley A. M., Thomas D. B. Structural alterations in the surface membrane during the cell cycle. J Cell Biol. 1971 Aug;50(2):562–564. doi: 10.1083/jcb.50.2.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips B., Roitt I. M. Evidence for transformation of human B lymphocytes by PHA. Nat New Biol. 1973 Feb 21;241(112):254–256. doi: 10.1038/newbio241254a0. [DOI] [PubMed] [Google Scholar]
- Schlossman S. F., Hudson L. Specific purification of lymphocyte populations on a digestible immunoabsorbent. J Immunol. 1973 Jan;110(1):313–319. [PubMed] [Google Scholar]
- Thomas D. B. Cyclic expression of blood group determinants in murine cells and their relationship to growth control. Nature. 1971 Oct 1;233(5318):317–321. doi: 10.1038/233317a0. [DOI] [PubMed] [Google Scholar]