Abstract
Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 106 neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85–90% cell death in 90 min, as measured by 51chromium release and dye exclusion. The mixture of granulocytes. monocytes, and lymphocytes present in an inflammatory exudate, and the intimate cell-to-cell association characteristic of cytotoxic phenomena may provide the in vivo requirements for such a system.
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- BOYSE E. A., OLD L. J., CHOUROULINKOV I. CYTOTOXIC TEST FOR DEMONSTRATION OF MOUSE ANTIBODY. Methods Med Res. 1964;10:39–47. [PubMed] [Google Scholar]
- Baehner R. L., Nathan D. G., Castle W. B. Oxidant injury of caucasian glucose-6-phosphate dehydrogenase-deficient red blood cells by phagocytosing leukocytes during infection. J Clin Invest. 1971 Dec;50(12):2466–2473. doi: 10.1172/JCI106747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Canty T. G., Wunderlich J. R. Quantitative in vitro assay of cytotoxic cellular immunity. J Natl Cancer Inst. 1970 Oct;45(4):761–772. [PubMed] [Google Scholar]
- Desser R. K., Himmelhoch S. R., Evans W. H., Januska M., Mage M., Shelton E. Guinea pig heterophil and eosinophil peroxidase. Arch Biochem Biophys. 1972 Feb;148(2):452–465. doi: 10.1016/0003-9861(72)90164-6. [DOI] [PubMed] [Google Scholar]
- Hubbard A. L., Cohn Z. A. The enzymatic iodination of the red cell membrane. J Cell Biol. 1972 Nov;55(2):390–405. doi: 10.1083/jcb.55.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchison D. J., Ittensohn O. L., Bjerregaard M. R. Growth of L1210 mouse leukemia cells in vitro. Exp Cell Res. 1966 Apr;42(1):157–170. doi: 10.1016/0014-4827(66)90328-4. [DOI] [PubMed] [Google Scholar]
- Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paul B., Sbarra A. J. The role of the phagocyte in host-parasite interactions. 13. The direct quantitative estimation of H2O2 in phagocytizing cells. Biochim Biophys Acta. 1968 Feb 1;156(1):168–178. doi: 10.1016/0304-4165(68)90116-5. [DOI] [PubMed] [Google Scholar]
- Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
- SCHULTZ J., KAMINKER K. Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization. Arch Biochem Biophys. 1962 Mar;96:465–467. doi: 10.1016/0003-9861(62)90321-1. [DOI] [PubMed] [Google Scholar]
- Steinman R. M., Cohn Z. A. The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro. J Cell Biol. 1972 Oct;55(1):186–204. doi: 10.1083/jcb.55.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zatti M., Rossi F., Patriarca P. The H2O2-production by polymorphonuclear leukocytes during phagocytosis. Experientia. 1968 Jul 15;24(7):669–670. doi: 10.1007/BF02138302. [DOI] [PubMed] [Google Scholar]
- van Furth R., Hirsch J. G., Fedorko M. E. Morphology and peroxidase cytochemistry of mouse promonocytes, monocytes, and macrophages. J Exp Med. 1970 Oct 1;132(4):794–812. doi: 10.1084/jem.132.4.794. [DOI] [PMC free article] [PubMed] [Google Scholar]