Abstract
Efforts to stimulate lymphocytes from measles seropositive and two patients with subacute sclerosing panencephalitis (SSPE) with either commercially available measles virus or virus isolated from a known case of SSPE failed to show any significant data using a microculture assay. Similar results were obtained using lymphocytes from two patients with active cytomegalovirus (CMV) infections and CMV seropositive individuals using CMV suspensions. On the other hand, lymphocytes from the patients with subacute sclerosing panencephalitis exhibited in vitro blastogenesis in culture with SSPE virus-infected HeLa cells. Similarly, lymphocytes from the CMV-infected patients demonstrated blastogenesis when cocultivated with CMV-infected WI-38 cells. This affords a new method for determining the cell-mediated immune capacity of patients with "slow" virus diseases.
Full Text
The Full Text of this article is available as a PDF (446.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Armstrong D., Balakrishnan S. L., Steger L., Yu B., Stenzel K. H. Cytomegalovirus infections with viremia following renal transplantation. Arch Intern Med. 1971 Jan;127(1):111–115. [PubMed] [Google Scholar]
- Armstrong D., Haghbin M., Balakrishnan S. L., Murphy M. L. Asymptomatic cytomegalovirus infection in children with leukemia. Am J Dis Child. 1971 Nov;122(5):404–407. doi: 10.1001/archpedi.1971.02110050074007. [DOI] [PubMed] [Google Scholar]
- Brown R. E., Madge G. E. Fatty acids and mitochondrial injury in Reye's syndrome. N Engl J Med. 1972 Apr 6;286(14):787–788. doi: 10.1056/NEJM197204062861427. [DOI] [PubMed] [Google Scholar]
- Glasgow L. A. Cellular immunity in host resistance to viral infections. Arch Intern Med. 1970 Jul;126(1):125–134. [PubMed] [Google Scholar]
- Horta-Barbosa L., Fuccillo D. A., London W. T., Jabbour J. T., Zeman W., Sever J. L. Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis. Proc Soc Exp Biol Med. 1969 Oct;132(1):272–277. doi: 10.3181/00379727-132-34196. [DOI] [PubMed] [Google Scholar]
- Horta-Barbosa L., Hamilton R., Wittig B., Fuccillo D. A., Sever J. L., Vernon M. L. Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science. 1971 Aug 27;173(3999):840–841. doi: 10.1126/science.173.3999.840. [DOI] [PubMed] [Google Scholar]
- Horta-Barbosa L., Krebs H., Ley A., Chen T. C., Gilkeson M. R., Sever J. L. Progressive increase in cerebrospinal fluid measles antibody levels in subacute sclerosing panencephalitis. Pediatrics. 1971 Apr;47(4):782–783. [PubMed] [Google Scholar]
- Immunologic abnormalities in subacute sclerosing panencephalitis. N Engl J Med. 1972 Apr 6;286(14):786–787. doi: 10.1056/NEJM197204062861426. [DOI] [PubMed] [Google Scholar]
- Jabbour J. T., Roane J. A., Sever J. L. Studies of delayed dermal hypersensitivity in patients with subacute sclerosing panencephalitis. Neurology. 1969 Oct;19(10):929–931. doi: 10.1212/wnl.19.10.929. [DOI] [PubMed] [Google Scholar]
- Rosenberg G. L., Farber P. A., Notkins A. L. In vitro stimulation of sensitized lymphocytes by herpes simplex virus and vaccinia virus. Proc Natl Acad Sci U S A. 1972 Mar;69(3):756–760. doi: 10.1073/pnas.69.3.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sell K. W., Thurman G. B., Ahmed A., Strong D. M. Plasma and spinal-fluid blocking factor in SSPE. N Engl J Med. 1973 Jan 25;288(4):215–216. doi: 10.1056/nejm197301252880418. [DOI] [PubMed] [Google Scholar]