Abstract
Anti-H-2 reagents have been tested on solubilized spleen cell preparations in combinations expected to be specific for D region products. Two different types of molecules were detected. One showed the expected reactivity with both antisera to private and antisera to public specificities. However, an additional molecule was detected which reacted only with antisera to public specificities. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis migration patterns indicated that both products have a similar molecular size of approximately 45,000 daltons. The data therefore present chemical evidence for the existence of a third H-2-associated gene product of 45,000 mol wt in addition to the classical H-2K and H-2D antigens.
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Selected References
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- Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
- Cullen S. E., Schwartz B. D., Nathenson S. G. The distribution of alloantigenic specificities of native H-2 products. J Immunol. 1972 Mar;108(3):596–600. [PubMed] [Google Scholar]
- Frelinger J. A., Murphy D. B., McCormick J. F. Tla types of H-2 congenic and recombinant mice. Transplantation. 1974 Sep;18(3):292–294. [PubMed] [Google Scholar]
- Hauptfeld V., Klein J. Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method. J Exp Med. 1975 Aug 1;142(2):288–298. doi: 10.1084/jem.142.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein J., Shreffler D. C. The H-2 model for the major histocompatibility systems. Transplant Rev. 1971;6:3–29. doi: 10.1111/j.1600-065x.1971.tb00457.x. [DOI] [PubMed] [Google Scholar]
- Rask L., Lindblom J. B., Peterson P. A. Structural and immunological similarities between HLA antigens from three loci. Eur J Immunol. 1976 Feb;6(2):93–100. doi: 10.1002/eji.1830060205. [DOI] [PubMed] [Google Scholar]
- Solheim B. G., Bratlie A., Sandberg L., Staub-Nielsen L., Thorsby E. Further evidence of a third HL-A locus. Tissue Antigens. 1973;3(6):439–453. doi: 10.1111/j.1399-0039.1973.tb00514.x. [DOI] [PubMed] [Google Scholar]
- Thorsby E. The human major histocompatibility system. Transplant Rev. 1974;18:51–129. [PubMed] [Google Scholar]