Abstract
Strains were constructed that contain mutational alterations affecting two distinct functional domains within the araC gene protein. The araCi (catabolite repression insensitivity) and araCh (catabolite repression hypersensitivity) mutations were used to alter the catabolite repression sensitivity domain, and mutation to D-fucose resistance was used to alter the inducer binding domain. araCh, D-fucose-resistant double mutants never exhibited constitutive ara operon expression, whereas all of the araCi, D-fucose-resistant double mutants did exhibit constitutivity. When L-arabinose was used as an inducer, most of the double mutants exhibited the sensitivity to catabolite repression associated with the araCi or araCh mutation. However, when D-fucose was used as an inducer, changes in sensitivity to catabolite repression were observed that were attributed to interactions between the two protein domains. The roles of catabolite activator protein and araC gene protein in the induction of the araBAD operon were discussed.
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Selected References
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- Bass R., Heffernan L., Sweadner K., Englesberg E. The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r. Arch Microbiol. 1976 Oct 11;110(1):135–143. doi: 10.1007/BF00416978. [DOI] [PubMed] [Google Scholar]
- Beverin S., Sheppard D. E., Park S. S. D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC. J Bacteriol. 1971 Jul;107(1):79–86. doi: 10.1128/jb.107.1.79-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colomé J., Wilcox G., Englesberg E. Constitutive mutations in the controlling site region of the araBAD operon of Escherichia coli B/r that decrease sensitivity to catabolite repression. J Bacteriol. 1977 Feb;129(2):948–958. doi: 10.1128/jb.129.2.948-958.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dickson R. C., Abelson J., Barnes W. M., Reznikoff W. S. Genetic regulation: the Lac control region. Science. 1975 Jan 10;187(4171):27–35. doi: 10.1126/science.1088926. [DOI] [PubMed] [Google Scholar]
- Englesberg E., Wilcox G. Regulation: positive control. Annu Rev Genet. 1974;8:219–242. doi: 10.1146/annurev.ge.08.120174.001251. [DOI] [PubMed] [Google Scholar]
- Gendron R. P., Sheppard D. E. Mutations in the L-arabinose operon of Escherichia coli B-r that result in hypersensitivity to catabolite repression. J Bacteriol. 1974 Feb;117(2):417–421. doi: 10.1128/jb.117.2.417-421.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heffernan L., Bass R., Englesberg E. Mutations affecting catabolite repression of the L-arabinose regulon in Escherichia coli B/r. J Bacteriol. 1976 Jun;126(3):1119–1131. doi: 10.1128/jb.126.3.1119-1131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heffernan L., Wilcox G. Effect of araC gene product on catabolite repression in the L-arabinose regulon. J Bacteriol. 1976 Jun;126(3):1132–1135. doi: 10.1128/jb.126.3.1132-1135.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopkins J. D. A new class of promoter mutations in the lactose operon of Escherichia coli. J Mol Biol. 1974 Aug 25;87(4):715–724. doi: 10.1016/0022-2836(74)90080-1. [DOI] [PubMed] [Google Scholar]
- MacInnes K. R., Sheppard D. E., Falgout B. Regulatory properties of araC(c) mutants in the L-arabinose operon of escherichia coliB/r. J Bacteriol. 1978 Jan;133(1):178–184. doi: 10.1128/jb.133.1.178-184.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ogata R. T., Gilbert W. An amino-terminal fragment of lac repressor binds specifically to lac operator. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851–5854. doi: 10.1073/pnas.75.12.5851. [DOI] [PMC free article] [PubMed] [Google Scholar]