Abstract
Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adherent cell, and does not require any adherent cell functions in our culture conditions. The ConA-dependent helper cells appear to be polyclonal in specificity. Thus, polyclonal stimulation of cytotoxicity by ConA requires T helper- T precursor collaboration in analogy to antigen-specific T helper-T precursor interactions. Unlike the antigen-specific interacitons, the ConA-driven cytotoxicity does not appear to require linked associative recognition for induction of cytotoxicity.
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Selected References
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- Bevan M. J., Cohn M. Cytotoxic effects of antigen- and mitogen-induced T cells on various targets. J Immunol. 1975 Feb;114(2 Pt 1):559–565. [PubMed] [Google Scholar]
- Bevan M. J., Langman R. E., Cohn M. H-2 antigen-specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency. Eur J Immunol. 1976 Mar;6(3):150–156. doi: 10.1002/eji.1830060303. [DOI] [PubMed] [Google Scholar]
- Clark W. R. An antigen-specific component of lectin-mediated cytotoxicity. Cell Immunol. 1975 Jun;17(2):505–516. doi: 10.1016/s0008-8749(75)80054-2. [DOI] [PubMed] [Google Scholar]
- Dutton R. W. Suppressor T cells. Transplant Rev. 1975;26:39–55. doi: 10.1111/j.1600-065x.1975.tb00174.x. [DOI] [PubMed] [Google Scholar]
- Forman J., Möller G. Generation of cytotoxic lymphocytes in mixed lymphocyte reactions. I. Specificity of the effector cells. J Exp Med. 1973 Sep 1;138(3):672–685. doi: 10.1084/jem.138.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jandinski J., Cantor H., Tadakuma T., Peavy D. L., Pierce C. W. Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1382–1390. doi: 10.1084/jem.143.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kagnoff M. F., Billings P., Cohn M. Functional characteristics of Peyer's patch lymphoid cells. II. Lipopolysaccharide is thymus dependent. J Exp Med. 1974 Feb 1;139(2):407–413. doi: 10.1084/jem.139.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee K. C., Shiozawa C., Shaw A., Diener E. Requirement for accessory cells in the antibody response to T cell-independent antigens in vitro. Eur J Immunol. 1976 Jan;6(1):63–68. doi: 10.1002/eji.1830060114. [DOI] [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. V. Target cells for tolerance induction. J Exp Med. 1970 Apr 1;131(4):675–699. doi: 10.1084/jem.131.4.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Möller G., Sjöberg O., Andersson J. Mitogen-induced lymphocyte-mediated cytotoxicity in vitro: effect of mitogens selectively activating T or B cells. Eur J Immunol. 1972 Dec;2(6):586–592. doi: 10.1002/eji.1830020621. [DOI] [PubMed] [Google Scholar]
- Ozato K., Ebert J. D. Concanavalin A potentiates syngeneic response in murine lymphocytes. J Exp Med. 1976 Jan 1;143(1):1–14. doi: 10.1084/jem.143.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ozato K., Ebert J. D. Concanavalin A potentiates syngeneic response in murine lymphocytes. J Exp Med. 1976 Jan 1;143(1):1–14. doi: 10.1084/jem.143.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peavy D. L., Pierce C. W. Cell-mediated immune responses in vitro. I. Suppression of the generation of cytotoxic lymphocytes by concanavalin A and concanavalin A-activated spleen cells. J Exp Med. 1974 Aug 1;140(2):356–369. doi: 10.1084/jem.140.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piguet P. F., Dewey H. K., Vassalli P. Synergistic and suppressive interactions among mouse T lymphocytes in the response to phytohemagglutinin. J Exp Med. 1975 Dec 1;142(6):1591–1599. doi: 10.1084/jem.142.6.1591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pilarski L. M. A requirement for antigen-specific helper T cells in the generation of cytotoxic T cells from thymocyte precursors. J Exp Med. 1977 Mar 1;145(3):709–725. doi: 10.1084/jem.145.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quintáns J., Lefkovits I. Clones of antibody-forming cells in pokeweed mitogen-stimulated microcultures. I. Requirements for the induction of the antibody response of nude spleen cells. Eur J Immunol. 1974 Sep;4(9):613–617. doi: 10.1002/eji.1830040907. [DOI] [PubMed] [Google Scholar]
- Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. II. Generation of a population of thymus-derived suppressor lymphocytes. J Exp Med. 1973 Mar 1;137(3):649–659. doi: 10.1084/jem.137.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stout R. D., Herzenberg L. A. The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. J Exp Med. 1975 Nov 1;142(5):1041–1051. doi: 10.1084/jem.142.5.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waterfield J. D., Waterfield E. M., Anaclerio A., Möller G. Lymphocyte-mediated cytotoxicity against tumor cells specificity and characterization of concanavalin A-activated cytotoxic effector lymphocytes. Transplant Rev. 1976;29:277–310. doi: 10.1111/j.1600-065x.1976.tb00205.x. [DOI] [PubMed] [Google Scholar]