Abstract
The effect of protein antigens on the locomotion of lymphocytes from the lymph nodes draining the site of antigenic challenge in immunized mice, and from the same nodes in control mice, was studied in filters using a checkerboard assay in which the absolute concentration and the concentration gradient of attractant was varied in a series of chambers. Serum albumin (HSA or BSA) was chemokinetic for unimmunized lymphocytes inasmuch as the distance migrated into filters by cells in its presence varied with the absolute concentration of albumin, but not with the concentration gradient, indicating an influence of the serum albumin on the rate but not on the direction of locomotion. Ovalbumin and nonalbumin proteins did not show this effect. Using the same assay, the migration of primed lymphocytes in the presence of the priming antigen was shown to be influenced by the antigen gradient in a way that suggested a positive chemotactic response of the lymphocytes to antigen. This response was only shown clearly when the cells were in a chemokinetic medium containing serum albumin.
Full Text
The Full Text of this article is available as a PDF (822.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson R., Glover A., Koornhof H. J., Rabson A. R. In vitro stimulation of neutrophil motility by levamisole: maintenance of cgmp levels in chemotactically stimulated levamisole-treated neutrophils. J Immunol. 1976 Aug;117(2):428–432. [PubMed] [Google Scholar]
- Jensen J. A., Esquenazi V. Chemotactic stimulation by cell surface immune reactions. Nature. 1975 Jul 17;256(5514):213–215. doi: 10.1038/256213a0. [DOI] [PubMed] [Google Scholar]
- Russell R. J., Wilkinson P. C., Sless F., Parrott D. M. Chemotaxis of lymphoblasts. Nature. 1975 Aug 21;256(5519):646–648. doi: 10.1038/256646a0. [DOI] [PubMed] [Google Scholar]
- Schreiner G. F., Unanue E. R. Anti-Ig-triggered movements of lymphocytes-specificity and lack of evidence for directional migration. J Immunol. 1975 Feb;114(2 Pt 2):809–814. [PubMed] [Google Scholar]
- Showell H. J., Freer R. J., Zigmond S. H., Schiffmann E., Aswanikumar S., Corcoran B., Becker E. L. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils. J Exp Med. 1976 May 1;143(5):1154–1169. doi: 10.1084/jem.143.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkinson P. C., Roberts J. A., Russell R. J., McLoughlin M. Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes. Clin Exp Immunol. 1976 Aug;25(2):280–287. [PMC free article] [PubMed] [Google Scholar]
- Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]