Abstract
We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of unprimed (BALB/c x C57BL/Ka)F1 mice to restore the adoptive primary anti-BSA and anti-DNP antibody responses. Purified populations of isotype-specific cells were prepared by immunofluorescent staining and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in complete Freund's adjuvant. Unfractionated spleen cells as well as IgM- and IgD- bearing cells restored the adoptive primary IgM and IgG antibody response. IgG-bearing cells restored a vigorous adoptive response which was all IgG (2-mercaptoethanol resistant). Depletion of IgG-bearing cells markedly increased the adoptive IgM response, and depletion of IgM-bearing cells markedly increased the IgG response. However, depletion of IgD-bearing cells resulted in a considerable reduction in the IgG response. The latter finding indicates that there is a subpopulation of IgD-bearing cells which express little or no surface IgM and which make a considerable contribution to the adoptive primary IgG response.
Full Text
The Full Text of this article is available as a PDF (600.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- Goodman S. A., Vitetta E. S., Melcher U., Uhr J. W. Cell surface immunoglobulin. XIII. Distribution of IgM and IgD-like molecules on small and large cells of mouse spleen. J Immunol. 1975 May;114(5):1646–1648. [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- Strober S., Dilley J. Maturation of B lymphocytes in the rat. I. Migration pattern, tissue distribution, and turnover rate of unprimed and primed B lymphocytes involved in the adoptive antidinitrophenyl response. J Exp Med. 1973 Dec 1;138(6):1331–1344. doi: 10.1084/jem.138.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strober S. Maturation of B lymphocytes in the rat. II. Subpopulations of virgin B lymphocytes in the spleen and thoracic duct lymph. J Immunol. 1975 Feb;114(2 Pt 2):877–885. [PubMed] [Google Scholar]
- Vitetta E. S., Uhr J. W. Immunoglobulin-receptors revisited. Science. 1975 Sep 19;189(4207):964–969. doi: 10.1126/science.1083069. [DOI] [PubMed] [Google Scholar]
- Zan-Bar I., Strober S., Vitetta E. S. The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. I. Surface immunoglobulin isotypes on primed B cells in the spleen. J Exp Med. 1977 May 1;145(5):1188–1205. doi: 10.1084/jem.145.5.1188. [DOI] [PMC free article] [PubMed] [Google Scholar]