Abstract
Mouse thymus, thymus-derived lymphocytes, and brain share an antigen determined by gene at the Thy-1 locus in chromosome 9 (1). Two alleles have been identified at this locus: Thy-1(a), coding for antigen Thy-1.1 (or θ-AKR) present in AKR and seven other strains; and Thy-1(b), coding for antigen Thy-1.2 (or{teta}-C3H) and present in C3H and all the remaining inbred strains. Injection of AKR thymocytes into inbred mice carrying the Thy-1(b) allele results in an immune response that can be measured either serologically by determining the level of antibodies in the recipients’ serum (1) or by counting plaque- forming cells (PFC) detectable in spleens of the recipients by means of an assay, with AKR thymocytes as target cells(2). The magnitude of PFC and serum antibody responses after a single thymocyte injection depends on the genetic make-up of the recipient. Three genes controlling the PFC response to the Thy- 1.1 antigen have been identified: Ir-Thy-1A and Ir-Thy-1B, which are closely linked to the major histocompatibility complex (H-2) of the mouse (3-6), and Ir-5, which is located at a distance of 17 cm to the right of the H-2 complex on chromosome 17 (6). Previous genetic mapping with H-2 recombinant strains has indicated that the two Ir-Thy-1 loci are located to the left of the IC subregion (7). Further experiments strongly suggested that either one or both Ir-Thy-1 loci map to the K rather than the I region of the H-2 complex (8). In this report, the study of an H- 2 mutant, CBA-H-2(ka) (M523) (9), and its parental strain, CBA/LacStoY (CBA) provided further evidence that one of these loci apparently resides in the K region and might even be identical with the H-2K locus in that region.
Full Text
The Full Text of this article is available as a PDF (363.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bach F. H., Widmer M. B., Segall M., Bach M. L., Klein J. Genetic and immunological complexity of major histocompatibility regions. Science. 1972 Jun 2;176(4038):1024–1027. doi: 10.1126/science.176.4038.1024. [DOI] [PubMed] [Google Scholar]
- Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
- Fuji H., Schultz R. T., Milgrom F. Cytolysis in agar of thymus cells by antibody-forming cells. Proc Soc Exp Biol Med. 1970 Jan;133(1):180–182. doi: 10.3181/00379727-133-34435. [DOI] [PubMed] [Google Scholar]
- Fuji H., Zaleski M., Milgrom F. Genetic control of immune response to theta-AKR alloantigen. J Immunol. 1972 Jan;108(1):223–230. [PubMed] [Google Scholar]
- Hauptfeld V., Hauptfeld M., Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed] [Google Scholar]
- Klein J. An attempt at an interpretation of the mouse H-2 complex. Contemp Top Immunobiol. 1976;5:297–336. [PubMed] [Google Scholar]
- Klein J., Forman J., Hauptfeld V., Egorov I. K. Immunogenetic analysis of H-2 mutations. III. Genetic mapping and involvement in immune reactions of the H-2ka mutation. J Immunol. 1975 Sep;115(3):716–718. [PubMed] [Google Scholar]
- Klein J., Hauptfeld M., Geib R., Hammerberg C. Immunogenetic analysis of H-2 mutations. V. Serological analysis of mutations H-2da, H-2ra, and H-2ka1. Transplantation. 1976 Dec;22(6):572–582. doi: 10.1097/00007890-197612000-00006. [DOI] [PubMed] [Google Scholar]
- Meo T., David C. S., Nabholz M., Miggiano V., Shreffler D. C. Demonstration by MLR test of a previously unsuspected intra-H-2 crossover in the B10.HTT strain: implications concerning location of MLR determinants in the Ir region. Transplant Proc. 1973 Dec;5(4):1507–1510. [PubMed] [Google Scholar]
- REIF A. E., ALLEN J. M. SPECIFICITY OF ISOANTISERA AGAINST LEUKAEMIC AND THYMIC LYMPHOCYTES. Nature. 1963 Dec 28;200:1332–1333. doi: 10.1038/2001332b0. [DOI] [PubMed] [Google Scholar]
- Schmitt-Verhulst A. M., Shearer G. M. Multiple H-2 linked immune response gene control of H-2 D-associated T-cell-mediated lympholysis to trinitrophenyl-modified autologous cells: Ir-like genes mapping to the left of I-A and within the I region. J Exp Med. 1976 Dec 1;144(6):1701–1706. doi: 10.1084/jem.144.6.1701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaleski M. B., Klein J. Immune response of mice to the Thy-1.1 antigen: intra-H-2 mapping of the complementary Ir-Thy-1 loci. J Immunol. 1976 Sep;117(3):814–817. [PubMed] [Google Scholar]
- Zaleski M., Fuji H., Milgrom F. Evidence for multigenic control of immune response to theta-AKR antigen in mice. Transplant Proc. 1973 Mar;5(1):201–204. [PubMed] [Google Scholar]
- Zaleski M., Klein J. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol. 1974 Oct;113(4):1170–1177. [PubMed] [Google Scholar]
- Zaleski M., Milgrom F. Complementary genes controlling immune response to theta-AKR antigen in mice. J Immunol. 1973 May;110(5):1238–1244. [PubMed] [Google Scholar]