Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1977 Jul 1;146(1):297–301. doi: 10.1084/jem.146.1.297

Ontogeny of Ia and IgD on IgM-bearing B lymphocytes in mice

PMCID: PMC2180751  PMID: 301548

Abstract

We used immunofluorescence to examine the developmental relationship of Ia and IgD on B cells. Pre-B cells in fetal liver did not express Ia. Only very few surface IgM-positive (sIgM+) B cells in fetal spleen were found to be Ia+ and were weakly stained for Ia. After birth there was a linear increase in the proportion of sIgM+ spleen cells which expressed Ia, reaching 95% by 9 days. Adult bone marrow also contains a sizeable proportion of sIgM+ Ia- cells. Unstimulated cells from fetal or newborn liver and spleen expressed Ia at the same rate in culture. Anti-Ia antisera suppressed the LPS-induced differentiation of IgM and IgG plasma cells in cultures of neonatal lymphocytes. Ia was also detected on IgM and IgG plasma cells in vitro suggesting that lipopolysaccharide (LPS)-stimulated B cells by may express Ia antigens, induced by LPS, or appearing as part of normal differentiation. IgD did not appear on sIgM+ cells until 3 days of age and then rose slowly to reach adult levels later than Ia antigens.

Full Text

The Full Text of this article is available as a PDF (354.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abney E. R., Hunter I. R., Parkhouse R. M. Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D. Nature. 1976 Feb 5;259(5542):404–406. doi: 10.1038/259404a0. [DOI] [PubMed] [Google Scholar]
  2. Abney E. R., Parkhouse R. M. Candidate for immunoglobulin D present on murine B lymphocytes. Nature. 1974 Dec 13;252(5484):600–602. doi: 10.1038/252600a0. [DOI] [PubMed] [Google Scholar]
  3. Benacerraf B., Katz D. H. The histocompatibility-linked immune response genes. Adv Cancer Res. 1975;21:121–173. doi: 10.1016/s0065-230x(08)60972-0. [DOI] [PubMed] [Google Scholar]
  4. Cooper M. D., Kearney J. F., Lawton A. R., Abney E. R., Parkhouse R. M., Preud'homme J. L., Seligmann M. Generation of immunoglobulin class diversity in b cells: a discussion with emphasis on idg development. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):573–581. [PubMed] [Google Scholar]
  5. Frelinger J. A., Neiderhuber J. E., David C. S., Shreffler D. C. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes. J Exp Med. 1974 Nov 1;140(5):1273–1284. doi: 10.1084/jem.140.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hämmerling U., Chin A. F., Abbott J. Ontogeny of murine B lymphocytes: sequence of B-cell differentiation from surface-immunoglobulin-negative precursors to plasma cells. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2008–2012. doi: 10.1073/pnas.73.6.2008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kearney J. F., Cooper M. D., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. III. Suppression of B cell maturation by anti-mouse immunoglobulin antibodies. J Immunol. 1976 Jun;116(6):1664–1668. [PubMed] [Google Scholar]
  8. Metcalf E. S., Klinman N. R. In vitro tolerance induction of neonatal murine B cells. J Exp Med. 1976 Jun 1;143(6):1327–1340. doi: 10.1084/jem.143.6.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Niederhuber J. E., Frelinger J. A., Dugan E., Coutinho A., Shreffler D. C. Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera. J Immunol. 1975 Dec;115(6):1672–1676. [PubMed] [Google Scholar]
  10. Nossal G. J., Pike B. L. Evidence for the clonal abortion theory of B-lymphocyte tolerance. J Exp Med. 1975 Apr 1;141(4):904–917. [PMC free article] [PubMed] [Google Scholar]
  11. Pierce S. K., Klinman N. R. The allogeneic bisection of carrier-specific enhancement of monoclonal B-cell responses. J Exp Med. 1975 Nov 1;142(5):1165–1179. doi: 10.1084/jem.142.5.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Press J. L., Klinman N. R. Enumeration and analysis of antibody-forming cell precursors in the neonatal mouse. J Immunol. 1973 Sep;111(3):829–835. [PubMed] [Google Scholar]
  13. Press J. L., Klinman N. R., McDevitt H. O. Expression of Ia antigens on hapten-specific B cells. I. Delineation of B-cell subpopulations. J Exp Med. 1976 Aug 1;144(2):414–427. doi: 10.1084/jem.144.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Raff M. C., Megson M., Owen J. J., Cooper M. D. Early production of intracellular IgM by B-lymphocyte precursors in mouse. Nature. 1976 Jan 22;259(5540):224–226. doi: 10.1038/259224a0. [DOI] [PubMed] [Google Scholar]
  15. Raff M. C., Owen J. J., Cooper M. D., Lawton A. R., 3rd, Megson M., Gathings W. E. Differences in susceptibility of mature and immature mouse B lymphocytes to anti-immunoglobulin-induced immunoglobulin suppression in vitro. Possible implications for B-cell tolerance to self. J Exp Med. 1975 Nov 1;142(5):1052–1064. doi: 10.1084/jem.142.5.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sachs D. H., Cone J. L. A mouse B-cell alloantigen determined by gene(s) linked to the major histocompatibility complex. J Exp Med. 1973 Dec 1;138(6):1289–1304. doi: 10.1084/jem.138.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sidman C. L., Unanue E. R. Receptor-mediated inactivation of early B lymphocytes. Nature. 1975 Sep 11;257(5522):149–151. doi: 10.1038/257149a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES