Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1977 Aug 1;146(2):561–570. doi: 10.1084/jem.146.2.561

The guinea pig I region. II. Functional analysis

PMCID: PMC2180755  PMID: 69005

Abstract

We have examined whether an association exists between specific Ia antigen genes and Ir genes which are encoded within the same haplotype. Functionally monospecific sera to the Ia antigens of the guinea pig MHC were selective in their ability to inhibit antigen-specific T-cell proliferation and we were thus able to demonstrate an association between individual Ia specificities and specific Ir genes. The results of these studies in inbred animals were confirmed by examining the association of Ir genes and Ia antigens in the outbred guinea pig population. Of great interest was the observation that antisera made against cross-reactive Ia antigens of strains lacking specific Ir genes would still inhibit immune responses of strains possessing the Ir gene, if the Ir gene was associated with that Ia antigen in the responder strain.

Full Text

The Full Text of this article is available as a PDF (826.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bluestein H. G., Green I., Benacerraf B. Specific immune response genes of the guinea pig. I. Dominant genetic control of immune responsiveness to copolymers of L-glutamic acid and L-alanine and L-glutamic acid and L-tyrosine. J Exp Med. 1971 Aug 1;134(2):458–470. doi: 10.1084/jem.134.2.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brondz B. D., Egorov I. K., Drizlikh G. I. Private specificities of H-2K and H-2D loci as possible selective targets for effector lymphocytes in cell-mediated immunity. J Exp Med. 1975 Jan 1;141(1):11–26. doi: 10.1084/jem.141.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dorf M. E., Maurer P. H., Merryman F., Benacerraf B. Inclusion group systems and cis-trans effects in responses controlled by the two complementing Ir-GLphi genes. J Exp Med. 1976 Apr 1;143(4):889–896. doi: 10.1084/jem.143.4.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Harrison M. R., Thurman G. B., Thomas G. M. A simple and versatile harvesting device for processing radioactive label incorporated into and-or released from cells in microculture. J Immunol Methods. 1974 Jan;3(1):11–16. doi: 10.1016/0022-1759(74)90027-1. [DOI] [PubMed] [Google Scholar]
  5. Janeway C. A., Jr, Paul W. E. Hapten-specific augmentation of the anti-idiotype antibody response to hapten-myeloma protein conjugates in mice. Eur J Immunol. 1973 Jun;3(6):340–347. doi: 10.1002/eji.1830030605. [DOI] [PubMed] [Google Scholar]
  6. Katz D. H., Graves M., Dorf M. E., Dimuzio H., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex. J Exp Med. 1975 Jan 1;141(1):263–268. doi: 10.1084/jem.141.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller J. F., Vadas M. A., Whitelaw A., Gamble J. Role of major histocompatibility complex gene products in delayed-type hypersensitivity. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2486–2490. doi: 10.1073/pnas.73.7.2486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rosenstreich D. L., Blake J. T., Rosenthal A. S. The peritoneal exudate lymphocyte. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs. J Exp Med. 1971 Nov 1;134(5):1170–1186. doi: 10.1084/jem.134.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schwartz B. D., Kask A. M., Paul W. E., Geczy A. F., Shevach E. M. The guinea pig I region. I. A structural and genetic analysis. J Exp Med. 1977 Aug 1;146(2):547–560. doi: 10.1084/jem.146.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shevach E. M., Paul W. E., Green I. Histocompatibility-linked immune response gene function in guinea pigs. Specific inhibition of antigen-induced lymphocyte proliferation by alloantisera. J Exp Med. 1972 Nov 1;136(5):1207–1221. doi: 10.1084/jem.136.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shevach E. M. The function of macrophages in antigen recognition by guinea pig T lymphocytes. III. Genetic analysis of the antigens mediating macrophage-T lymphocyte interaction. J Immunol. 1976 May;116(5):1482–1489. [PubMed] [Google Scholar]
  13. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  14. Stupp Y., Paul W. E., Benacerraf B. Structural control of immunogenicity. II. Antibody synthesis and cellular immunity in response to immunization with mono-epsilon-oligo-L-lysines. Immunology. 1971 Oct;21(4):583–594. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES