Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1977 Aug 1;146(2):623–628. doi: 10.1084/jem.146.2.623

Cytotoxic T lymphocytes induced against allogeneic I-region determinants react with Ia molecules on trinitrophenyl-conjugated syngeneic target cells

P Billings, S Burakoff, ME Dorf, B Benacerraf
PMCID: PMC2180769  PMID: 69009

Abstract

The major histocompatibility complex codes for determinants which are recognized by and serve as targets for cytolytic T lymphocytes (CTL) (1). Antigens coded for by the K and D loci of the H-2 complex can activate xenogeneic or allogeneic CTL (2,3). In addition, the H-2K or H-2D gene products function as those molecules against which syngeneic CTL responses specific for chemical, viral, and minor H antigens are directed (4-8). It has recently been shown that Ia determinants can also serve as target antigens for distinct but weaker CTL responses (9-13). Those clones which recognize Ia antigens see them independently of K- or D- coded antigens as shown in genetic studies and by antisera-blocking experiments (12,13). We have proposed that the existence of clones of CTL specific for I-region-coded determinants is not fortuitous; rather these clones specifically recognize Ia determinants and may have an immunoregulatory role. These CTL may affect those immune functions which are at least partially dependent on or controlled by I-region-coded molecules. Two predictions can be made and tested concerning the role of Ia determinants in cytolytic systems and the role, if any, of I-region- specific CTL in regulating the immune response: (a) that if as we and others have shown, certain Ia specificities can serve as a third series of major histocompatibility antigens, then Ia antigens should be susceptible to the same types of antigenic modifications as H-2K- or H-2D-coded structures and thus serve as targets for CTL directed against modified-self in selected systems; and (b) that allogeneically induced I-region-specific CTL should demonstrate cross-reactivity with targets bearing modified syngeneic I-region-coded determinants. Data will be present which demonstrates that trinitrophenyl (TNP)-modified syngeneic I-region determinants can serve as targets for CTL induced by allogeneic Ia antigens.

Full Text

The Full Text of this article is available as a PDF (461.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Bach M. L., Sondel P. M. Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature. 1976 Jan 29;259(5541):273–281. doi: 10.1038/259273a0. [DOI] [PubMed] [Google Scholar]
  2. Bevan M. J. Interaction antigens detected by cytotoxic T cells with the major histocompatibility complex as modifier. Nature. 1975 Jul 31;256(5516):419–421. doi: 10.1038/256419a0. [DOI] [PubMed] [Google Scholar]
  3. Billings P., Burakoff S., Dorf M. E., Benacerraf B. Cytotoxic T lymphocytes specific for I region determinants do not require interactions with H-2K or D gene products. J Exp Med. 1977 May 1;145(5):1387–1392. doi: 10.1084/jem.145.5.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burakoff S. J., Dorf M. E., Benacerraf B. Specificity of rat xenogeneic cell-mediated cytolysis for the products of the K and D loci of the mouse H-2 complex. J Immunol. 1977 May;118(5):1795–1798. [PubMed] [Google Scholar]
  5. Burakoff S. J., Germain R. N., Benacerraf B. Cross-reactive lysis of trinitrophenyl (TNP)-derivatized H-2 incompatible target cells by cytolytic T lymphocytes generated against syngeneic TNP spleen cells. J Exp Med. 1976 Dec 1;144(6):1609–1620. doi: 10.1084/jem.144.6.1609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burakoff S. J., Germain R. N., Dorf M. E., Benacerrah B. Inhibition of cell-mediated cytolysis of trinitrophenyl-derivatized target cells by alloantisera directed to the products of the K and D loci of the H-2 complex. Proc Natl Acad Sci U S A. 1976 Feb;73(2):625–629. doi: 10.1073/pnas.73.2.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  8. Gordon R. D., Simpson E., Samelson L. E. In vitro cell-mediated immune responses to the male specific(H-Y) antigen in mice. J Exp Med. 1975 Nov 1;142(5):1108–1120. doi: 10.1084/jem.142.5.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein J., Chiang C. L., Hauptfeld V. Histocompatibility antigens controlled by the I region of the murine H-2 complex. II. K/D region compatibility is not required for I-region cell-mediated lymphocytotoxicity. J Exp Med. 1977 Feb 1;145(2):450–454. doi: 10.1084/jem.145.2.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein J., Geib R., Chiang C., Hauptfeld V. Histocompatibility antigens controlled by the I region of the murine H-2 complex. I. Mapping of H-2A and H-2C loci. J Exp Med. 1976 Jun 1;143(6):1439–1452. doi: 10.1084/jem.143.6.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nabholz M., Young H., Rynbeek A., Boccardo R., David C. S., Meo T., Miggiano V., Shreffler D. C. I-region-associated determinants: expression on mitogen-stimulated lymphocytes and detection by cytotoxic T cells. Eur J Immunol. 1975 Sep;5(9):594–599. doi: 10.1002/eji.1830050903. [DOI] [PubMed] [Google Scholar]
  12. Peck A. B., Alter B. J., Lindahl K. F. Specificity in T cell mediated lympholysis: identical genetic control of the proliferative and effector phases of allogeneic and xenogeneic reactions. Transplant Rev. 1976;29:189–221. doi: 10.1111/j.1600-065x.1976.tb00202.x. [DOI] [PubMed] [Google Scholar]
  13. Shearer G. M., Rehn T. G., Schmitt-Verhulst A. M. Role of the murine major histocompatibility complex in the specificity of in vitro T-cell-mediated lympholysis against chemically-modified autologous lymphocytes. Transplant Rev. 1976;29:222–246. doi: 10.1111/j.1600-065x.1976.tb00203.x. [DOI] [PubMed] [Google Scholar]
  14. Wagner H., Götze D., Ptschelinzew L., Röllinghoff M. Induction of cytotoxic T lymphocytes against I-region-coded determinants: in vitro evidence for a third histocompatibility locus in the mouse. J Exp Med. 1975 Dec 1;142(6):1477–1487. doi: 10.1084/jem.142.6.1477. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES